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Phylogenetic tree analysis.
| العنوان: | Phylogenetic tree analysis. |
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| المؤلفون: | Samuel Christopher Katru, Anand Setty Balakrishnan, Gnanasekar Munirathinam, Azadeh Hadadianpour, Scott A. Smith, Ramaswamy Kalyanasundaram |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Medicine, Microbiology, Cell Biology, Genetics, Molecular Biology, Biotechnology, Evolutionary Biology, Immunology, Developmental Biology, Cancer, Mental Health, Infectious Diseases, Virology, Computational Biology, Physical Sciences not elsewhere classified, tropical parasitic infection, clinical filarial infections, cdna expression library, blast analysis showed, bancrofti may play, wuchereria bancrofti <, |
| الوصف: | The PCR products of the DNA insert from the bacteriophages were sequenced at the UIC Sanger sequencing facility. The nucleotide sequences obtained from all 15 clones were subjected to NCBI BLAST analysis. This identified two proteins, Bm2855 (GenBank, Gene ID 6099714), which was not annotated previously and W . bancrofti Translationally Controlled Tumor Protein (TCTP), which was previously described from our laboratory [ 30 ]. Based on the CLUSTAL Omega multiple sequence alignment data of Bm2855, we constructed a phylogenetic tree. Our results showed that Bm2855 of B . malayi and W . bancrofti share over 95% sequence homology. Bm2855 homologue protein were also present in several nematodes. However, there is no Bm2855 homologous protein in the humans. Given the significant homology in several nematodes and the fact that the Bm2855 protein is recognized by IgE antibodies, the sequence was named as Nematode Pan Allergen (NPA) and deposited in the NCBI GenBank with accession number ON023112. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Phylogenetic_tree_analysis_/25221815 |
| DOI: | 10.1371/journal.pntd.0011972.g002 |
| الاتاحة: | https://doi.org/10.1371/journal.pntd.0011972.g002 https://figshare.com/articles/figure/Phylogenetic_tree_analysis_/25221815 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.322B8F38 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pntd.0011972.g002 |
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