Academic Journal
A specific calprotectin neo-epitope (CPa9-HNE) in serum from inflammatory bowel disease patients is associated with neutrophil activity and endoscopic severity
| العنوان: | A specific calprotectin neo-epitope (CPa9-HNE) in serum from inflammatory bowel disease patients is associated with neutrophil activity and endoscopic severity |
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| المؤلفون: | Mortensen, Joachim Høg, Sinkeviciute, Divuke, Manon-Jensen, Tuba, Domislović, Viktor, McCall, Kathryn, Thudium, Christian S., Brinar, Marko, Önnerfjord, Patrik, Goodyear, Carl S., Krznarić, Željko, Karsdal, Morten Asser, Bay-Jensen, Anne-Christine |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2022 |
| المجموعة: | University of Glasgow: Enlighten - Publications |
| الوصف: | Background and aims: Endoscopy and the use of fecal calprotectin (fecal CP) are among the least favored methods for assessing disease activity by inflammatory bowel disease (IBD) patients; the handling/processing of fecal samples is also impractical. Therefore, we sought to develop a novel neo-epitope serum calprotectin ELISA, CPa9-HNE, with the aim of quantifying neutrophil activity and NETosis and proposing a non-invasive method for monitoring disease activity in IBD patients. Methods: In vitro cleavage was performed by mixing calprotectin (S100A9/S100A8) with human neutrophil elastase (HNE), and a novel HNE-derived calprotectin neo-epitope (CPa9-HNE) was identified by mass spectrometry for ELISA development. The CPa9-HNE ELISA was quantified in supernatants from ex vivo activated neutrophils and serum samples from patients with ulcerative colitis (UC, n = 43), Crohn’s disease (CD, n = 93), and healthy subjects (HS, n = 23). For comparison, fecal CP and MRP8/14 biomarkers were also measured. Results: CPa9-HNE was specific for activated neutrophils ex vivo. Serum CPa9-HNE levels were four fold higher in CD (p<0.0001) and UC (p<0.0001) patients than in HS. CPa9-HNE correlated well with the SES-CD score (r=0.61, p<0.0001), MES (r=0.46, p=0.0141), and the full Mayo score (r=0.52, p=0.0013). CPa9-HNE was able to differentiate between CD and UC patients in endoscopic remission and moderate/severe disease activity (CD: AUC=0.82 (p=0.0003), UC: AUC=0.87 (p=0.0004). The performance of CPa9-HNE was equipotent or slightly better than that of fecal CP. Conclusion: Serum CPa9-HNE levels were highly associated with CD and UC patients. CPa9-HNE correlated with the SES-CD score and the full Mayo score, indicating a strong association with disease activity. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://eprints.gla.ac.uk/268047/1/268047.pdf; Mortensen, J. H. et al. (2022) A specific calprotectin neo-epitope (CPa9-HNE) in serum from inflammatory bowel disease patients is associated with neutrophil activity and endoscopic severity. Journal of Crohn's and Colitis , 16(9), pp. 1447-1460. (doi:10.1093/ecco-jcc/jjac047 ) (PMID:35304895) (PMCID:PMC9455793) |
| الاتاحة: | https://eprints.gla.ac.uk/268047/ https://eprints.gla.ac.uk/268047/1/268047.pdf |
| Rights: | cc_by_nc_4 |
| رقم الانضمام: | edsbas.3208B739 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |