Academic Journal
REPORT In vivo activity of nuclease-resistant siRNAs
| العنوان: | REPORT In vivo activity of nuclease-resistant siRNAs |
|---|---|
| المؤلفون: | Juliana M. Layzer, Anton P. Mccaffrey, Alice K. Tanner, Zan Huang, Mark A. Kay, Bruce A. Sullenger |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://kaylab.stanford.edu/manuscripts/RNA-04-layzer.pdf. |
| المجموعة: | CiteSeerX |
| مصطلحات موضوعية: | RNAi, siRNA, modified nucleotides, luciferase |
| الوصف: | Chemical modifications have been incorporated into short interfering RNAs (siRNAs) without reducing their ability to inhibit gene expression in mammalian cells grown in vitro. In this study, we begin to assess the potential utility of 2�-modified siRNAs in mammals. We demonstrate that siRNA modified with 2�-flouro (2�-F) pyrimidines are functional in cell culture and have a greatly increased stability and a prolonged half-life in human plasma as compared to 2�-OH containing siRNAs. Moreover, we show that the 2�-F containing siRNAs are functional in mice and can inhibit the expression of a target gene in vivo. However, even though the modified siRNAs have greatly increased resistance to nuclease degradation in plasma, this increase in stability did not translate into enhanced or prolonged inhibitory activity of target gene reduction in mice following tail vein injection. Thus, this study shows that 2�-F modified siRNAs are functional in vivo, but that they are not necessarily more potent than unmodified siRNAs in animals. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.1361; http://kaylab.stanford.edu/manuscripts/RNA-04-layzer.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.419.1361 http://kaylab.stanford.edu/manuscripts/RNA-04-layzer.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.31D1F24C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |