Academic Journal
Myelin basic protein stimulates plasminogen activation via tissue plasminogen activator following binding to independent L-lysine-containing domains
| العنوان: | Myelin basic protein stimulates plasminogen activation via tissue plasminogen activator following binding to independent L-lysine-containing domains |
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| المؤلفون: | Gonzàlez Gronow, Mario, Fiedler Temer, Jenny, Farías Gómez, Cristian, Wang, Fang, Ray, Rupa, Ferrell, Paul D., Pizzo, Salvatore V. |
| المصدر: | Biochemical and Biophysical Research Communications |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2017 |
| المجموعة: | Universidad de Chile: Repositorio académico |
| مصطلحات موضوعية: | Myelin basic protein, Plasminogen, Plasminogen receptor, Tissue plasminogen activator (tPA) |
| الوصف: | Myelin basic protein (MBP) is a key component of myelin, the specialized lipid membrane that encases the axons of all neurons. Both plasminogen (Pg) and tissue-type plasminogen activator (t-PA) bind to MBP with high affinity. We investigated the kinetics and mechanisms involved in this process using immobilized MBP and found that Pg activation by t-PA is significantly stimulated by MBP. This mechanism involves the binding of t-PA via a lysine-dependent mechanism to the Lys91 residue of the MBP NH2-terminal region Asp82 -Pro99, and the binding of Pg via a lysine-dependent mechanism to the Lys122 residue of the MBP COOH-terminal region Leu109-Gly126. In this context, MBP mimics fibrin and because MBP is a plasmin substrate, our results suggest direct participation of the Pg activation system on MBP physiology. ; Fondo Nacional de Desarrollo Cientίfico y Tecnolόgico de Chile, FONDECYT No 1130451 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Biochemical and Biophysical Research Communications 490 (2017) 855-860; http://dx.doi.org/10.1016/j.bbrc.2017.06.131; http://repositorio.uchile.cl/handle/2250/149113 |
| DOI: | 10.1016/j.bbrc.2017.06.131 |
| الاتاحة: | https://doi.org/10.1016/j.bbrc.2017.06.131 http://repositorio.uchile.cl/handle/2250/149113 |
| Rights: | Attribution-NonCommercial-NoDerivs 3.0 Chile ; http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ |
| رقم الانضمام: | edsbas.31D11266 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.bbrc.2017.06.131 |
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