Academic Journal
CotC-CotU Heterodimerization during Assembly of the Bacillus subtilis Spore Coat
| العنوان: | CotC-CotU Heterodimerization during Assembly of the Bacillus subtilis Spore Coat |
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| المؤلفون: | Isticato, Rachele, Pelosi, Assunta, Zilhão, Rita, Baccigalupi, Loredana, Henriques, Adriano O., De Felice, Maurilio, Ricca, Ezio |
| المصدر: | Journal of Bacteriology ; volume 190, issue 4, page 1267-1275 ; ISSN 0021-9193 1098-5530 |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2008 |
| الوصف: | We report evidence that CotC and CotU, two previously identified components of the Bacillus subtilis spore coat, are produced concurrently in the mother cell chamber of the sporulating cell under the control of σ K and GerE and immediately assembled around the forming spore. In the coat, the two proteins interact to form a coat component of 23 kDa. The CotU-CotC interaction was not detected in two heterologous hosts, suggesting that it occurs only in B. subtilis . Monomeric forms of both CotU and CotC failed to be assembled at the surface of the developing spore and accumulated in the mother cell compartment of cells mutant for cotE . In contrast, neither CotU nor CotC accumulated in the mother cell compartment of cells mutant for cotH . These results suggest that CotH is required to protect both CotU and CotC in the mother cell compartment of the sporangium and that CotE is needed to allow their assembly and subsequent interaction at the spore surface. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jb.01425-07 |
| DOI: | 10.1128/JB.01425-07 |
| الاتاحة: | http://dx.doi.org/10.1128/jb.01425-07 https://journals.asm.org/doi/pdf/10.1128/JB.01425-07 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.31994E07 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jb.01425-07 |
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