Academic Journal
Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates
| العنوان: | Characterization of Actinobacterial Communities from Arauca River Sediments (Colombia) Reveals Antimicrobial Potential Presented in Low Abundant Isolates |
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| المؤلفون: | Arango, Carolina, Acosta Gonzalez, Alejandro, Parra Giraldo, Claudia M., Sánchez Quitian, Zilpa A., Kerr, Russell, Díaz Barrera, Luis Eduardo |
| المصدر: | Universidad de La Sabana ; Intellectum Repositorio Universidad de La Sabana |
| بيانات النشر: | The Open Microbiology Journal |
| سنة النشر: | 2018 |
| المجموعة: | Repositorio Universidad de La Sabana |
| مصطلحات موضوعية: | Actinobacteria, Antimicrobial, Bioprospecting, MALDI-TOF MS, Physicochemical pretreatments, Taxonomic dereplication |
| الوصف: | 7 páginas ; Introduction: New strategies have been arisen to set a rapid and effective screening for selection of microorganism with bioactive potential. This study suggests that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents. Material and Methods: An unstudied microbial community from a tropical river sediment in Colombian Orinoquía is described, applying an extended cultivation strategy using physicochemical pretreatments, biological screenings and taxonomic dereplication through MALDI-TOF MS approach. Results: Actinobacteria-like isolates (790) were growth and their antimicrobial activity was assessed against methicillin-resistant Staphylococcus aureus, Vancomycin-resistant Enterococcus faecium, extended-spectrum β-lactamase Klebsiella pnumoniae, and clinical isolates of Cladosporium cladosporioides and Epicoccum nigrum. Seventy-eight isolates, belonging to the Streptomycetaceae family according to 16S rDNA analysis were found to have antimicrobial activity and were categorized as low abundance actinobacteria by MALDI-TOF MS. Conclusion: The results suggest that combination of physicochemical pretreatments and taxonomic dereplication of microbial collections through MALDI-TOF MS, facilitates the detection of low abundance actinobacteria with potential as a source of antimicrobial agents. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Open Microbiol J. 2018;12: 181–194; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997859/; http://hdl.handle.net/10818/42362 |
| DOI: | 10.2174/1874285801812010181 |
| الاتاحة: | http://hdl.handle.net/10818/42362 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997859/ https://doi.org/10.2174/1874285801812010181 |
| Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0/ ; openAccess |
| رقم الانضمام: | edsbas.3181D817 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2174/1874285801812010181 |
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