Academic Journal
Poster 364: Tendon-Bone Interface Healing Improvement by Moderate Treadmill Exercise Following an Anterior Cruciate Ligament Reconstruction in a Murine Model
| العنوان: | Poster 364: Tendon-Bone Interface Healing Improvement by Moderate Treadmill Exercise Following an Anterior Cruciate Ligament Reconstruction in a Murine Model |
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| المؤلفون: | Okazaki, Yuki, Carballo, Camila, Piacentini, Alexander, Morita, Wataru, Ying, Liang, Takata, Yasushi, Retzky, Julia, Suzuki, Yuki, Chen, Tony, Rodeo, Scott |
| المصدر: | Orthopaedic Journal of Sports Medicine ; volume 12, issue 7_suppl2 ; ISSN 2325-9671 2325-9671 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2024 |
| الوصف: | Objectives: Recent studies have been using the anterior cruciate ligament reconstruction (ACLR) mouse model to study the tendon-bone healing process and the development of post-traumatic osteoarthritis. While it is known that mechanical load has an important effect on ACL graft healing, a limited number of studies have investigated the impact of treadmill exercise. No study has yet examined the effects of different exercise regimens following an ACLR in mice. The purpose of this study is to explore the effect of different mechanical loading stimuli on the tendon-bone interface (TBI) healing process following ACLR in mice. We hypothesized that moderate loading would positively affect TBI healing after ACLR. Methods: The study protocol was approved by our Institutional Animal Care and Use Committee (IACUC 2020-0024). A total of 58 C57BL/6 (12 weeks old) male mice underwent to an ACL reconstruction in the right knee as previously described. The first 30 mice were assigned into 5 different exercise groups (n=6 per group) and used to evaluate different exercise regimens (Study Arm 1- Fig. 1A). After identifying the optimal exercise regimen, an additional 28 mice were included in the study (Study arm 2 - Fig. 1B). All mice were acclimated on the treadmill following a 5-day acclimation protocol prior to surgery. Starting 7 days post-surgery, the treadmill exercise was performed 5 days/week for a total of 3 weeks. All animals were euthanized at 4 weeks post-surgery. Micro-CT imaging was performed using a Scanco mCT 45 system (Scanco Medical). The bone tunnel was identified, a region of interest (ROI) was defined as the bone tunnel diameter (0.64mm), and bone volume fraction (bone volume/total volume), bone mineral density, and trabecular number were evaluated in the tibial tunnel. Biomechanical Testing was performed in a materials-testing machine (EnduraTEC ELF 3200) (Fig. 2). The knee joints were carefully dissected to isolate the ACL graft. Custom fixtures were designed to ensure that the ACL was aligned in the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/2325967124s00330 |
| DOI: | 10.1177/2325967124S00330 |
| الاتاحة: | https://doi.org/10.1177/2325967124s00330 https://journals.sagepub.com/doi/pdf/10.1177/2325967124S00330 |
| Rights: | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.307AF128 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/2325967124s00330 |
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