Academic Journal
Development of a liquid chromatographic method for the determination of related substances and assay of D-cycloserine
| العنوان: | Development of a liquid chromatographic method for the determination of related substances and assay of D-cycloserine |
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| المؤلفون: | Pendela, Murali Mohan, Dragovic, Sanja, Bockx, Lien, Hoogmartens, Jos, Van Schepdael, Ann, Adams, Erwin |
| بيانات النشر: | Pergamon-elsevier science ltd |
| سنة النشر: | 2008 |
| المجموعة: | KU Leuven: Lirias |
| مصطلحات موضوعية: | d-cycloserine, liquid chromatography, related substances, assay |
| الوصف: | D-cycloserine or D-4-amino-3-isoxazolidinone is an antibiotic produced by Streptomyces garyphalus and Streptomyces orchidaceus. D-cycloserine is used in the second line treatment of tuberculosis and is often used in developing countries. Therefore, expensive high-tech techniques are not recommended for analysis. Here, a liquid chromatography method with ultraviolet detection (LC-UV) is described using a base deactivated column (Hypersil BDS column; 25 cm x 4.6 mm I.D.) kept at 45 degrees C. The gradient method uses mobile phases containing acetonitrile (ACN), 20 mM sodium octane sulphonate (SOS), 0.2 M potassium dihydrogen phosphate buffer pH 2.8, water: A: (4:70:10:16 v/v/v/v) and 13: (17:70:10:3 v/v/v/v). The method proved to be robust, linear, repeatable, sensitive, selective and easy to perform. For the related substances test 50 mu l of a 0.5 mg/ml D-cycloserine solution is injected. For assay, a concentration of 0.1 mg/ml is proposed to avoid overloading of the detector. (C) 2008 Elsevier B.V. All rights reserved. ; status: published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0731-7085 |
| Relation: | Journal of pharmaceutical and biomedical analysis vol:47 issue:4-5 pages:807-811; https://lirias.kuleuven.be/handle/123456789/196918; http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DOISource&SrcApp=PRODUCT_NAME&KeyAID=10.1016%2Fj.jpba.2008.03.012&DestApp=DOI&SrcAppSID=APP_SID&SrcJTitle=WURS_TITLE |
| الاتاحة: | https://lirias.kuleuven.be/handle/123456789/196918 http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=DOISource&SrcApp=PRODUCT_NAME&KeyAID=10.1016%2Fj.jpba.2008.03.012&DestApp=DOI&SrcAppSID=APP_SID&SrcJTitle=WURS_TITLE |
| رقم الانضمام: | edsbas.3059A9E2 |
| قاعدة البيانات: | BASE |
| تدمد: | 07317085 |
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