التفاصيل البيبلوغرافية
| العنوان: |
Volumetric imaging of the tumor microvasculature reflects outcomes and genomic states of clear cell renal cell carcinoma |
| المؤلفون: |
Kaneko, Yuta, Masuda, Tsukasa, Takamatsu, Kimiharu, Mikami, Shuji, Nakamura, Kohei, Nishihara, Hiroshi, Mizuno, Ryuichi, Tanaka, Nobuyuki, Oya, Mototsugu |
| المساهمون: |
Japan Society for the Promotion of Science, Princess Takamatsu Cancer Research Fund, Takeda Science Foundation |
| المصدر: |
The Journal of Pathology: Clinical Research ; volume 10, issue 4 ; ISSN 2056-4538 2056-4538 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2024 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Tumor structure is heterogeneous and complex, and it is difficult to obtain complete characteristics by two‐dimensional analysis. The aim of this study was to visualize and characterize volumetric vascular information of clear cell renal cell carcinoma (ccRCC) tumors using whole tissue phenotyping and three‐dimensional light‐sheet microscopy. Here, we used the diagnosing immunolabeled paraffin‐embedded cleared organs pipeline for tissue clearing, immunolabeling, and three‐dimensional imaging. The spatial distributions of CD34, which targets blood vessels, and LYVE‐1, which targets lymphatic vessels, were examined by calculating three‐dimensional density, vessel length, vessel radius, and density curves, such as skewness, kurtosis, and variance of the expression. We then examined those associations with ccRCC outcomes and genetic alteration state. Formalin‐fixed paraffin‐embedded tumor samples from 46 ccRCC patients were included in the study. Receiver operating characteristic curve analyses revealed the associations between blood vessel and lymphatic vessel distributions and pathological factors such as a high nuclear grade, large tumor size, and the presence of venous invasion. Furthermore, three‐dimensional imaging parameters stratified ccRCC patients regarding survival outcomes. An analysis of genomic alterations based on volumetric vascular information parameters revealed that PI3K‐mTOR pathway mutations related to the blood vessel radius were significantly different. Collectively, we have shown that the spatial elucidation of volumetric vasculature information could be prognostic and may serve as a new biomarker for genomic alterations. High‐end tissue clearing techniques and volumetric immunohistochemistry enable three‐dimensional analysis of tumors, leading to a better understanding of the microvascular structure in the tumor space. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/2056-4538.12388 |
| الاتاحة: |
http://dx.doi.org/10.1002/2056-4538.12388 |
| Rights: |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.2FE2B36C |
| قاعدة البيانات: |
BASE |