Academic Journal

Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in Fragaria vesca Genotypes of Differing Cold Tolerance

التفاصيل البيبلوغرافية
العنوان: Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in Fragaria vesca Genotypes of Differing Cold Tolerance
المؤلفون: Isam Fattash, Zachary Deitch, Relindis Njah, Nelson Osuagwu, Vera Mageney, Robert C. Wilson, Jahn Davik, Muath Alsheikh, Stephen Randall
المصدر: International Journal of Molecular Sciences; Volume 22; Issue 11; Pages: 6124
بيانات النشر: Multidisciplinary Digital Publishing Institute
سنة النشر: 2021
المجموعة: MDPI Open Access Publishing
مصطلحات موضوعية: dehydrins, ABA-regulated genes, CBF genes, alcohol dehydrogenase
جغرافية الموضوع: agris
الوصف: Identifying and characterizing cold responsive genes in Fragaria vesca associated with or responsible for low temperature tolerance is a vital part of strawberry cultivar development. In this study we have investigated the transcript levels of eight genes, two dehydrin genes, three putative ABA-regulated genes, two cold–inducible CBF genes and the alcohol dehydrogenase gene, extracted from leaf and crown tissues of three F. vesca genotypes that vary in cold tolerance. Transcript levels of the CBF/DREB1 transcription factor FvCBF1E exhibited stronger cold up-regulation in comparison to FvCBF1B.1 in all genotypes. Transcripts of FvADH were highly up-regulated in both crown and leaf tissues from all three genotypes. In the ‘ALTA’ genotype, FvADH transcripts were significantly higher in leaf than crown tissues and more than 10 to 20-fold greater than in the less cold-tolerant ‘NCGR1363’ and ‘FDP817’ genotypes. FvGEM, containing the conserved ABRE promoter element, transcript was found to be cold-regulated in crowns. Direct comparison of the kinetics of transcript and protein accumulation of dehydrins was scrutinized. In all genotypes and organs, the changes of XERO2 transcript levels generally preceded protein changes, while levels of COR47 protein accumulation preceded the increases in COR47 RNA in ‘ALTA’ crowns.
نوع الوثيقة: text
وصف الملف: application/pdf
اللغة: English
Relation: Molecular Genetics and Genomics; https://dx.doi.org/10.3390/ijms22116124
DOI: 10.3390/ijms22116124
الاتاحة: https://doi.org/10.3390/ijms22116124
Rights: https://creativecommons.org/licenses/by/4.0/
رقم الانضمام: edsbas.2F5BA736
قاعدة البيانات: BASE