Academic Journal
Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody
| العنوان: | Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody |
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| المؤلفون: | Randall, Richard Edward, Young, Dan Frushard, Pisliakova, Maria, Andrejeva, Jelena, West, Lynsey, Rossler, Luis, Morath, Volker, Hughes, David John, Goodbourn, Steve |
| المصدر: | Randall , R E , Young , D F , Pisliakova , M , Andrejeva , J , West , L , Rossler , L , Morath , V , Hughes , D J & Goodbourn , S 2025 , ' Single-cycle parainfluenza virus type 5 vectors for producing recombinant proteins, including a humanized anti-V5 tag antibody ' , Journal of General Virology , vol. 106 , no. 1 . https://doi.org/10.1099/jgv.0.002061 |
| سنة النشر: | 2025 |
| مصطلحات موضوعية: | Anti-V5-antibody, Expression vectors, Parainfluenza virus type 5, Persistence |
| الوصف: | Parainfluenza virus type 5 (PIV5) can cause either persistent or acute/lytic infections in a wide range of mammalian tissue culture cells. Here, we have generated PIV5 fusion (F)-expressing helper cell lines that support the replication of F-deleted viruses. As proof of the principle that F-deleted single-cycle infectious viruses can be used as safe and efficient expression vectors, we have cloned and expressed a humanized (Hu) version of the mouse anti-V5 tag antibody (clone SV5-Pk1). We show that multiple different cell lines can be infected and express high levels of the Hu anti-V5 antibody, with Chinese hamster ovary cells expressing 20–50 mg l−1 after 5 days when cells were grown to a density of ~1×106 cells per millilitre at the time of infection. We suggest that PIV5-based vectors may be further developed to produce recombinant proteins both in vitro and in vivo . |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.1099/jgv.0.002061 |
| الاتاحة: | https://research-portal.st-andrews.ac.uk/en/publications/bc80e3c3-841b-403a-b781-2aef3af135ae https://doi.org/10.1099/jgv.0.002061 https://research-repository.st-andrews.ac.uk/bitstream/10023/31156/1/Randall_2025_JoGV_Single-cycle-parainfluenza-virus-type-5-vectors-producing-recombinant-proteins_CC.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.2EFD1D46 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1099/jgv.0.002061 |
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