Dissertation/ Thesis
Structural and functonal analysis of membrane protein complexes from the photosynthetic bacterium Chlorobaculum tepidum ; Δομική και λειτουργική ανάλυση μεμβρανικών πρωτεϊνικών συμπλόκων από το φωτοσυνθετικό βακτήριο Chlorobaculum tepidum
| العنوان: | Structural and functonal analysis of membrane protein complexes from the photosynthetic bacterium Chlorobaculum tepidum ; Δομική και λειτουργική ανάλυση μεμβρανικών πρωτεϊνικών συμπλόκων από το φωτοσυνθετικό βακτήριο Chlorobaculum tepidum |
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| المؤلفون: | Lyratzakis, Alexandros, Λυρατζάκης, Αλέξανδρος |
| بيانات النشر: | University of Crete (UOC) Πανεπιστήμιο Κρήτης |
| سنة النشر: | 2024 |
| المجموعة: | National Archive of PhD Theses (National Documentation Centre Greece) |
| مصطلحات موضوعية: | Κρυογενική ηλεκτρονική μικροσκοπία, Πυρηνικός μαγνητικός συντονισμός, Φασματομετρία μαζών υψηλής διακριτικής ικανότητας (HRMS), Πρωτεομική ανάλυση, Μεταβολομική ανάλυση, ΓΚΡΟΜΑΞ, Ατομιστικές προσομοιώσεις μοριακής δυναμικής, Cryo-EM, NMR, Mass spectrometry, Proteomics, Metabolomics, GROMCS, Molecular dynamic simulations, Χημεία, Φυσικές Επιστήμες, διεπιστημονική προσέγγιση, Chemical Sciences, Natural Sciences, Chemistry, interdisciplinary |
| الوصف: | Chlorobaculum tepidum (Cba tepidum) is a green sulfur phototroph bacterium that predominantly oxidizes reduced sulfur compounds for photoautotrophic growth. Cba tepidum, can oxidize sulfide and thiosulfate to produce extracellular S0 globules, which can be further oxidized to sulfate and used as electron donors for phototrophic growth. To gain insight into its sulfur metabolism, label-free quantitative proteomics was utilized. The sampling consisted of cell lysates from different metabolic states, including a sulfur production state (10 h post-incubation), the beginning of sulfur consumption (20 h), and the end of sulfur consumption (40 h), respectively. The results showed that Cba tepidum regulates enzymes involved in sulfur metabolism in response to the availability of reduced sulfur compounds and a model showing the protein expression is presented in this study. The biosynthesis of polyphosphate (polyP) granule is important for survival for bacteria during resistance to diverse environmental stresses but the biogenesis of the polyP granule is poorly understood. In Cba tepidum the presence of electron rich granules was reported, but no further information was provided. Based on the label free proteomic data it was possible to track down the differential expression of the polyP related enzymes. Finally, the structural characterization and the final proof of the existence of the polyphosphate polymer and the total phosphate content in the cells was achieved by P31 NMR and absorption spectroscopy respectively. Phototrophic organism converts light energy from the sun into chemical energy, thereby sustaining most life forms on Earth. Light energy is captured by antenna systems and is subsequently transferred to the reaction center (RC) to initiate a series of charge-separation and electron-transfer reactions to the terminal electron acceptors. Chlorosomes, the light-harvesting apparatus of green bacteria, are unique antenna systems in which pigments are organized in aggregates rather than associated with proteins. ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | English |
| Relation: | http://hdl.handle.net/10442/hedi/58035 |
| الاتاحة: | http://hdl.handle.net/10442/hedi/58035 |
| رقم الانضمام: | edsbas.2EC5BA95 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |