Academic Journal
New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition
| العنوان: | New human papilloma virus E2 transcription factor mimics: A tripyrrole-peptide conjugate with tight and specific DNA-recognition |
|---|---|
| المؤلفون: | Wetzler, D.E., Comin, M.J., Krajewski, K., Gallo, M. |
| المصدر: | PLoS ONE 2011;6(7) |
| سنة النشر: | 2011 |
| المجموعة: | Repositorio Digital Institucional - Universidad de Buenos Aires (RDI UBA) |
| مصطلحات موضوعية: | distamycin A, DNA, peptide, protein E2, transcription factor, unclassified drug, antivirus agent, biomimetic material, DNA binding protein, E2 protein, Human papillomavirus type 16, oncoprotein, peptide fragment, pyrrole derivative, virus DNA, alpha helix, article, circular dichroism, computer model, conjugation, DNA synthesis, gel mobility shift assay, nonhuman, nuclear magnetic resonance spectroscopy, protein DNA binding, protein interaction, protein structure, amino acid sequence, chemical structure, chemistry |
| الوصف: | Background: Human papillomavirus (HPV) is the main causative agent of cervical cancer, particularly high risk strains such us HPV-16, -18 and -31. The viral encoded E2 protein acts as a transcriptional modulator and exerts a key role in viral DNA replication. Thus, E2 constitutes an attractive target for developing antiviral agents. E2 is a homodimeric protein that interacts with the DNA target through an α-helix of each monomer. However, a peptide corresponding to the DNA recognition helix of HPV-16 E2 binds DNA with lower affinity than its full-length DNA binding domain. Therefore, in an attempt to promote the DNA binding of the isolated peptide, we have designed a conjugate compound of the E2 α-helix peptide and a derivative of the antibiotic distamycin, which involves simultaneous minor- and major-groove interactions. Methodology/Principal Findings: An E2 α-helix peptide-distamycin conjugate was designed and synthesized. It was characterized by NMR and CD spectroscopy, and its DNA binding properties were investigated by CD, DNA melting and gel shift experiments. The coupling of E2 peptide with distamycin does not affect its structural properties. The conjugate improves significantly the affinity of the peptide for specific DNA. In addition, stoichiometric amounts of specific DNA increase meaningfully the helical population of the peptide. The conjugate enhances the DNA binding constant 50-fold, maintaining its specificity. Conclusions/Significance: These results demonstrate that peptide-distamycin conjugates are a promising tool to obtain compounds that bind the E2 target DNA-sequences with remarkable affinity and suggest that a bipartite major/minor groove binding scaffold can be a useful approach for therapeutic treatment of HPV infection. © 2011 Wetzler et al. ; Fil:Wetzler, D.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Comin, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Gallo, M. Universidad de Buenos Aires. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | http://hdl.handle.net/20.500.12110/paper_19326203_v6_n7_p_Wetzler; http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_19326203_v6_n7_p_Wetzler_oai |
| الاتاحة: | https://hdl.handle.net/20.500.12110/paper_19326203_v6_n7_p_Wetzler http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_19326203_v6_n7_p_Wetzler_oai |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/2.5/ar |
| رقم الانضمام: | edsbas.2E5C2BF9 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |