Academic Journal
In vitro and intracellular activities of frog skin temporins against Legionella pneumophila and its eukaryotic hosts
| العنوان: | In vitro and intracellular activities of frog skin temporins against Legionella pneumophila and its eukaryotic hosts |
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| المؤلفون: | Crépin, Alexandre, Jégou, Jean-Francois, André, Sonia, Ecale, Florine, Croitoru, Anastasia, Cantereau, Anne, Berjeaud, Jean-Marc, Ladram, Ali, Verdon, Julien |
| المساهمون: | Écologie et biologie des interactions (EBI Poitiers ), Université de Poitiers = University of Poitiers (UP)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Inflammation, Tissus épithéliaux et Cytokines EA 4331 (LITEC Poitiers ), Université de Poitiers = University of Poitiers (UP), Biosynthèse des Signaux Peptidiques IBPS (IBPS-BIOSIPE), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Toxicité environnementale, cibles thérapeutiques, signalisation cellulaire (T3S - UMR_S 1124), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire d'Optique et Biosciences (LOB), École polytechnique (X), Institut Polytechnique de Paris (IP Paris)-Institut Polytechnique de Paris (IP Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Signalisation et Transports Ioniques Membranaires (STIM Poitiers ), Université de Poitiers = University of Poitiers (UP)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the French Center of Scientific Research (CNRS), the University of Poitiers and the EBI laboratory. A part of this work was also supported by funds from Sorbonne University. |
| المصدر: | ISSN: 2045-2322. |
| بيانات النشر: | CCSD Nature Publishing Group |
| سنة النشر: | 2020 |
| المجموعة: | Université François-Rabelais de Tours: HAL |
| مصطلحات موضوعية: | [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| الوصف: | International audience ; Temporin-SHa (SHa) is a small cationic host defence peptide (HDP) produced in skin secretions of the Sahara frog Pelophylax saharicus. This peptide has a broad-spectrum activity, efficiently targeting bacteria, parasites and viruses. Noticeably, SHa has demonstrated an ability to kill Leishmania infantum parasites (amastigotes) within macrophages. Recently, an analog of SHa with an increased net positive charge, named [K3]SHa, has been designed to improve those activities. SHa and [K3]SHa were both shown to exhibit leishmanicidal activity mainly by permeabilization of cell membranes but could also induce apoptotis-like death. Temporins are usually poorly active against Gram-negative bacteria whereas many of these species are of public health interest. Among them, Legionella pneumophila, the etiological agent of Legionnaire's disease, is of major concern. Indeed, this bacterium adopts an intracellular lifestyle and replicate inside alveolar macrophages likewise inside its numerous protozoan hosts. Despite several authors have studied the antimicrobial activity of many compounds on L. pneumophila released from host cells, nothing is known about activity on intracellular L. pneumophila within their hosts, and subsequently mechanisms of action that could be involved. Here, we showed for the first time that SHa and [K3]SHa were active towards several species of Legionella. Both peptides displayed bactericidal activity and caused a loss of the bacterial envelope integrity leading to a rapid drop in cell viability. Regarding amoebae and THP-1-derived macrophages, SHa was less toxic than [K3]SHa and exhibited low half maximal lethal concentrations (LC50). When used at non-toxic concentration (6.25 µM), SHa killed more than 90% L. pneumophila within amoebae and around 50% within macrophages. Using SHa labeled with the fluorescent dye Cy5, we showed an evenly diffusion within cells except in vacuoles. Moreover, SHa was able to enter the nucleus of amoebae and accumulate in the nucleolus. This ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32132569; PUBMED: 32132569 |
| DOI: | 10.1038/s41598-020-60829-2 |
| الاتاحة: | https://inserm.hal.science/hal-02505077 https://inserm.hal.science/hal-02505077v1/document https://inserm.hal.science/hal-02505077v1/file/s41598-020-60829-2.pdf https://doi.org/10.1038/s41598-020-60829-2 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2E5AE385 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-020-60829-2 |
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