Academic Journal
CDC20B is required for deuterosome-mediated centriole production in 1 multiciliated cells
| العنوان: | CDC20B is required for deuterosome-mediated centriole production in 1 multiciliated cells |
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| المؤلفون: | Revinski, Diego, R, Zaragosi, Laure-Emmanuelle, Boutin, Camille, Deprez, Marie, Thomé, Virginie, Rosnet, Olivier, Gay, Anne-Sophie, Paquet, Agnès, Pons, Nicolas, Ponzio, Gilles, Marcet, Brice, Kodjabachian, Laurent, Barbry, Pascal |
| المساهمون: | Institut de Biologie du Développement de Marseille (IBDM), Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes (IPC), Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011) |
| المصدر: | ISSN: 2041-1723. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2018 |
| المجموعة: | HAL Université Côte d'Azur |
| مصطلحات موضوعية: | multiciliated cell, centriole, deuterosome, motile cilia, CDC20B, Separase, PLK1, 16 cell cycle, Xenopus, mouse, human, [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | International audience ; Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene, which hosts microRNA-449abc. We show that CDC20B protein associates to deuterosomes and is required for centriole release and subsequent cilia production in mouse and Xenopus MCCs. CDC20B interacts with PLK1, a kinase known to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescues centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-02064869; https://hal.science/hal-02064869; https://hal.science/hal-02064869/document; https://hal.science/hal-02064869/file/Revinski%20et%20al%202018_PMID30405130_HAL.pdf |
| DOI: | 10.1038/s41467-018-06768-z |
| الاتاحة: | https://hal.science/hal-02064869 https://hal.science/hal-02064869/document https://hal.science/hal-02064869/file/Revinski%20et%20al%202018_PMID30405130_HAL.pdf https://doi.org/10.1038/s41467-018-06768-z |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2E4F9EC8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41467-018-06768-z |
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