التفاصيل البيبلوغرافية
| العنوان: |
Reconstructing Soma–Soma Synapse-like Vesicular Exocytosis with DNA Origami |
| المؤلفون: |
Jiangbo Liu (1911988), Min Li (12799), Fan Li (49953), Zhilei Ge (1844305), Qian Li (114642), Mengmeng Liu (501348), Jiye Shi (631279), Lihua Wang (14548), Xiaolei Zuo (1481800), Chunhai Fan (9209), Xiuhai Mao (2042866) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Cell Biology, Genetics, Neuroscience, Immunology, Hematology, Physical Sciences not elsewhere classified, membrane vesicle exchange, intercellular communications, soma, junction, synapse-like, carbon fiber nanometric electrodes, study synaptic communications, DNA origami nanostructure-based method |
| الوصف: |
Cell–cell communications exhibit distinct physiological functions in immune responses and neurotransmitter signaling. Nevertheless, the ability to reconstruct a soma–soma synapse-like junction for probing intercellular communications remains difficult. In this work, we develop a DNA origami nanostructure-based method for establishing cell conjugation, which consequently facilitates the reconstruction of a soma–soma synapse-like junction. We demonstrate that intercellular communications including small molecule and membrane vesicle exchange between cells are maintained in the artificially designed synapse-like junction. By inserting the carbon fiber nanometric electrodes into the soma–soma synapse-like junction, we accomplish the real-time monitoring of individual vesicular exocytotic events and obtain the information on vesicular exocytosis kinetics via analyzing the parameters of current spikes. This strategy provides a versatile platform to study synaptic communications. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Reconstructing_Soma_Soma_Synapse-like_Vesicular_Exocytosis_with_DNA_Origami/15066650 |
| DOI: |
10.1021/acscentsci.1c00645.s001 |
| الاتاحة: |
https://doi.org/10.1021/acscentsci.1c00645.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.2DF98F6A |
| قاعدة البيانات: |
BASE |