Academic Journal
Knockout of circRNAs by base editing back-splice sites of circularized exons
| العنوان: | Knockout of circRNAs by base editing back-splice sites of circularized exons |
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| المؤلفون: | Gao, Xiang, Ma, Xu-Kai, Li, Xiang, Li, Guo-Wei, Liu, Chu-Xiao, Zhang, Jun, Wang, Ying, Wei, Jia, Chen, Jia, Chen, Ling-Ling, Yang, Li |
| المساهمون: | National Natural Science Foundation of China, Ministry of Science and Technology of the People's Republic of China, Howard Hughes Medical Institute, China Postdoctoral Science Foundation, Shanghai Municipal Human Resources and Social Security Bureau |
| المصدر: | Genome Biology ; volume 23, issue 1 ; ISSN 1474-760X |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2022 |
| الوصف: | Many circular RNAs (circRNAs) are produced from back-splicing of exons of precursor mRNAs and are generally co-expressed with cognate linear RNAs. Methods for circRNA-specific knockout are lacking, largely due to sequence overlaps between forms. Here, we use base editors (BEs) for circRNA depletion. By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress circular and linear RNAs. Targeting sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular but not linear RNAs. As hundreds of exons are predominantly back-spliced to produce circRNAs, this provides an efficient method to deplete circRNAs for functional study. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s13059-021-02563-0 |
| DOI: | 10.1186/s13059-021-02563-0.pdf |
| DOI: | 10.1186/s13059-021-02563-0/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s13059-021-02563-0 https://link.springer.com/content/pdf/10.1186/s13059-021-02563-0.pdf https://link.springer.com/article/10.1186/s13059-021-02563-0/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.2DB6A47C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13059-021-02563-0 |
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