Academic Journal
NADH-independent enzymatic assay to quantify extracellular and intracellular L-lactate levels
| العنوان: | NADH-independent enzymatic assay to quantify extracellular and intracellular L-lactate levels |
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| المؤلفون: | Bouchez, Cyrielle, Daubon, Thomas, Mourier, Arnaud |
| المساهمون: | Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 2666-1667 ; STAR Protocols ; https://hal.science/hal-04284913 ; STAR Protocols, 2022, 3 (2), pp.101403. ⟨10.1016/j.xpro.2022.101403⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2022 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | Lactate is a central metabolite in energy metabolism and is also involved in cell signaling and epigenetic regulations. Here, we describe an NADH-independent enzymatic assay allowing rapid, selective, and sensitive quantification of L-lactate down to the pmol range. We detail lactate extraction from intracellular and extracellular fractions, followed by total protein amount determination and enzymatic assay. This approach allows quantification of intracellular and extracellular L-lactate levels, validated by treating adherent and non-adherent cells with inhibitors of lactate transporters (MCT). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04284913; https://hal.science/hal-04284913; https://hal.science/hal-04284913/document; https://hal.science/hal-04284913/file/1-s2.0-S2666166722002830-main.pdf |
| DOI: | 10.1016/j.xpro.2022.101403 |
| الاتاحة: | https://hal.science/hal-04284913 https://hal.science/hal-04284913/document https://hal.science/hal-04284913/file/1-s2.0-S2666166722002830-main.pdf https://doi.org/10.1016/j.xpro.2022.101403 |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2D71E29C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.xpro.2022.101403 |
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