Academic Journal
Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō
| العنوان: | Probiotic properties of Bacillus subtilis DG101 isolated from the traditional Japanese fermented food nattō |
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| المؤلفون: | Cecilia Leñini, Facundo Rodriguez Ayala, Anibal Juan Goñi, Liliana Rateni, Akira Nakamura, Roberto Ricardo Grau |
| المصدر: | Frontiers in Microbiology, Vol 14 (2023) |
| بيانات النشر: | Frontiers Media S.A. |
| سنة النشر: | 2023 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | probiotics, fermented soybean, nattō, Bacillus subtilis DG101, beneficial biofilms, metal bioremediation, Microbiology, QR1-502 |
| الوصف: | Spore-forming probiotic bacteria offer interesting properties as they have an intrinsic high stability, and when consumed, they are able to survive the adverse conditions encountered during the transit thorough the host gastrointestinal (GI) tract. A traditional healthy food, nattō, exists in Japan consisting of soy fermented by the spore-forming bacterium Bacillus subtilis natto. The consumption of nattō is linked to many beneficial health effects, including the prevention of high blood pressure, osteoporosis, and cardiovascular-associated disease. We hypothesize that the bacterium B. subtilis natto plays a key role in the beneficial effects of nattō for humans. Here, we present the isolation of B. subtilis DG101 from nattō and its characterization as a novel spore-forming probiotic strain for human consumption. B. subtilis DG101 was non-hemolytic and showed high tolerance to lysozyme, low pH, bile salts, and a strong adherence ability to extracellular matrix proteins (i.e., fibronectin and collagen), demonstrating its potential application for competitive exclusion of pathogens. B. subtilis DG101 forms robust liquid and solid biofilms and expresses several extracellular enzymes with activity against food diet-associated macromolecules (i.e., proteins, lipids, and polysaccharides) that would be important to improve food diet digestion by the host. B. subtilis DG101 was able to grow in the presence of toxic metals (i.e., chromium, cadmium, and arsenic) and decreased their bioavailability, a feature that points to this probiotic as an interesting agent for bioremediation in cases of food and water poisoning with metals. In addition, B. subtilis DG101 was sensitive to antibiotics commonly used to treat infections in medical settings, and at the same time, it showed a potent antimicrobial effect against pathogenic bacteria and fungi. In mammalians (i.e., rats), B. subtilis DG101 colonized the GI tract, and improved the lipid and protein serum homeostasis of animals fed on the base of a normal- or a deficient-diet ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1664-302X |
| Relation: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1253480/full; https://doaj.org/toc/1664-302X; https://doaj.org/article/3c6a2c61d50c46a98cd9949a6996da15 |
| DOI: | 10.3389/fmicb.2023.1253480 |
| الاتاحة: | https://doi.org/10.3389/fmicb.2023.1253480 https://doaj.org/article/3c6a2c61d50c46a98cd9949a6996da15 |
| رقم الانضمام: | edsbas.2D0E66C6 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1664302X |
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| DOI: | 10.3389/fmicb.2023.1253480 |