التفاصيل البيبلوغرافية
| العنوان: |
Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability |
| المؤلفون: |
González Díaz, Aida, Carrera Salinas, Anna, Pinto, Miguel, Cubero, Meritxell, Ende, Arie van der, Langereis, Jeroen D., Domínguez, M. Ángeles, Ardanuy, Carmen, Bajanca Lavado, Paula, Marti, Sara |
| المصدر: |
Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
| بيانات النشر: |
Springer Science and Business Media LLC |
| سنة النشر: |
2022 |
| المجموعة: |
Dipòsit Digital de la Universitat de Barcelona |
| مصطلحات موضوعية: |
Bacils, Genòmica, Bacillus (Bacteria), Genomics |
| الوصف: |
Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
10 p.; application/pdf |
| اللغة: |
English |
| تدمد: |
2045-2322 |
| Relation: |
Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-07185-5; Scientific Reports, 2022, vol 12, num 1; https://doi.org/10.1038/s41598-022-07185-5; http://hdl.handle.net/2445/184448 |
| الاتاحة: |
http://hdl.handle.net/2445/184448 |
| Rights: |
cc by (c) Gonzalez Diaz, Aida et al, 2022 ; http://creativecommons.org/licenses/by/3.0/es/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.2CFFEDBB |
| قاعدة البيانات: |
BASE |
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