Academic Journal
Assessing Bioprinted Functionalized Grafts for Biological Tendon Augmentation In Vitro
| العنوان: | Assessing Bioprinted Functionalized Grafts for Biological Tendon Augmentation In Vitro |
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| المؤلفون: | Cristina Del Amo, Miguel Perez-Garrastachu, Ines Jauregui, Xabier Llama-Pino, Isabel Andia |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 9, p 4752 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | extrusion bioprinting, tendon, grafts, platelet-rich plasma, inflammation, angiogenesis, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Tendinopathy, characterized by inflammatory and degenerative changes, presents challenges in sports and medicine. In addressing the limitations of conservative management, this study focuses on developing tendon grafts using extrusion bioprinting with platelet-rich plasma (PRP)-infused hydrogels loaded with tendon cells. The objective is to understand paracrine interactions initiated by bioprinted tendon grafts in either inflamed or non-inflamed host tissues. PRP was utilized to functionalize methacrylate gelatin (GelMA), incorporating tendon cells for graft bioprinting. Bioinformatic analyses of overexpressed proteins, predictive of functional enrichment, revealed insights into PRP graft behavior in both non-inflamed and inflamed environments. PRP grafts activated inflammatory pathways, including Interleukin 17 (IL-17), neuroinflammation, Interleukin 33 (IL-33), and chemokine signaling. Interleukin 1 beta (IL-1b) in the graft environment triggered p38 mitogen-activated protein kinase (MAPK) signaling, nuclear factor kappa light chain enhancer of activated B cells (NF-kB) canonical pathway, and Vascular Endothelial Growth Factor (VEGF) signaling. Biological enrichment attributed to PRP grafts included cell chemotaxis, collagen turnover, cell migration, and angiogenesis. Acellular PRP grafts differed from nude grafts in promoting vessel length, vessel area, and junction density. Angiogenesis in cellular grafts was enhanced with newly synthesized Interleukin 8 (IL-8) in cooperation with IL-1b. In conclusion, paracrine signaling from PRP grafts, mediated by chemokine activities, influences cell migration, inflammation, and angiogenic status in host tissues. Under inflammatory conditions, newly synthesized IL-8 regulates vascularization in collaboration with PRP. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/9/4752; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/6b6cf52185024fa3ad3b8bbf28c223cc |
| DOI: | 10.3390/ijms25094752 |
| الاتاحة: | https://doi.org/10.3390/ijms25094752 https://doaj.org/article/6b6cf52185024fa3ad3b8bbf28c223cc |
| رقم الانضمام: | edsbas.2CB0FB0A |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25094752 |