Academic Journal
Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis
| العنوان: | Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis |
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| المؤلفون: | Akil, Caner, Tran, Linh T., Orhant-Prioux, Magali, Baskaran, Yohendran, Senju, Yosuke, Takeda, Shuichi, Chotchuang, Phatcharin, Muengsaen, Duangkamon, Schulte, Albert, Manser, Edward, Blanchoin, Laurent, Robinson, Robert C. |
| المصدر: | Communications Biology |
| بيانات النشر: | Nature Portfolio |
| سنة النشر: | 2022 |
| المجموعة: | Okayama University Scientific Achievement Repository / 岡山大学学術成果リポジトリ |
| الوصف: | Charting the emergence of eukaryotic traits is important for understanding the characteristics of organisms that contributed to eukaryogenesis. Asgard archaea and eukaryotes are the only organisms known to possess regulated actin cytoskeletons. Here, we determined that gelsolins (2DGels) from Lokiarchaeota (Loki) and Heimdallarchaeota (Heim) are capable of regulating eukaryotic actin dynamics in vitro and when expressed in eukaryotic cells. The actin filament severing and capping, and actin monomer sequestering, functionalities of 2DGels are strictly calcium controlled. We determined the X-ray structures of Heim and Loki 2DGels bound actin monomers. Each structure possesses common and distinct calcium-binding sites. Loki2DGel has an unusual WH2-like motif (LVDV) between its two gelsolin domains, in which the aspartic acid coordinates a calcium ion at the interface with actin. We conclude that the calcium-regulated actin cytoskeleton predates eukaryogenesis and emerged in the predecessors of the last common ancestor of Loki, Heim and Thorarchaeota. Calcium-regulated actin filament assembly predates eukaryogenesis and was present in the last common ancestor of Asgard archaea Loki, Heim, and Thorarchaeota. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2399-3642 |
| Relation: | info:pmid/36045281; https://ousar.lib.okayama-u.ac.jp/files/public/6/63891/20220912133847482658/fulltext.pdf; https://ousar.lib.okayama-u.ac.jp/63891 |
| الاتاحة: | https://ousar.lib.okayama-u.ac.jp/files/public/6/63891/20220912133847482658/fulltext.pdf https://ousar.lib.okayama-u.ac.jp/63891 |
| Rights: | © The Author(s) 2022 |
| رقم الانضمام: | edsbas.2C9F8ED3 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 23993642 |
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