التفاصيل البيبلوغرافية
| العنوان: |
Expression of some genes implicated in antioxidative defense system in sunflower infected with broomrape |
| المؤلفون: |
Duca, M.V., Mutu Calmîş, A., Mutu, A., Tabără, O., Martea, R.I. |
| المصدر: |
Life sciences in the dialogue of generations: connections between universities, academia and business community |
| سنة النشر: |
2019 |
| الوصف: |
Orobanche cumana Wallr. broomrape is an obligate and nonphotosynthetic root parasitic angiosperms that cause severe agricultural problems in many crops of sunflower. It is widespread in Europe and Asia, where it can cause over 80% crop losses. In the host-parasite system, the first defense response of the plant is the rapid production of reactive oxygen species ROS that induce the oxidative stress. ROS are the result of the partial reduction of atmospheric O2 and they can oxidize multiple cellular components like proteins and lipids, DNA and RNA, causing ultimately cell death. In order to cope, the plants keep ROS under control by an efficient enzymatic oxygen scavenging system. Many methods of O. cumana control are available with more or less efficiency but genetic resistance remains of the most efficient method.nbsp; Thus, the aim of this study was the evaluation of transcriptional activitiy of 15 genes involved in antioxidant defense against oxidative stress during infection with broomrape in dynamics 2, 6, 12 and 24 hours after infestation. The experimental stage was performed in Petri dishes with perlite substrate. For evaluation of relative expression, RNA samples were obtained from the infected sunflower roots and quantified by realtime PCR.nbsp; Three sunflower genotypes, two resistant hybrids Favorit and PR64LE20 and one susceptible Performer to O. cumana was used in this study. The transcriptional activity of ROS ndash; scaverging genes was influenced by infection with broomrape, up-regulated 43% cases or was down-regulated 57% cases.nbsp; The analysis of total 15 genes showed different expression profiles between susceptible and resistant genotypes. Comparative to resistant genotypes in the susceptible genotype Performer the majority of genes were down-regulated. For the majority of genes the resistant genotypes were up-regulated at 12 hours after infestation.nbsp; This study are supported by the National Agency for Research and Development of the Republic of Moldova [project for young researchers ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://ibn.idsi.md/vizualizare_articol/89472 |
| الاتاحة: |
https://ibn.idsi.md/vizualizare_articol/89472 |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.2C477E29 |
| قاعدة البيانات: |
BASE |