Academic Journal
Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice ; Biodistribution rapide d'OMV (Outer-Membrane Vesicles) de l'intestin vers des organes via le sang chez la souris
| العنوان: | Rapid Biodistribution of Fluorescent Outer-Membrane Vesicles from the Intestine to Distant Organs via the Blood in Mice ; Biodistribution rapide d'OMV (Outer-Membrane Vesicles) de l'intestin vers des organes via le sang chez la souris |
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| المؤلفون: | Schaack, Béatrice, Mercier, Corinne, Katby, Maya, Hannani, Dalil, Vollaire, Julien, Robert, Julie Suzanne, Caffaratti, Clément, Blanquet, Françoise, Nicoud, Olivier, Josserand, Véronique, Laurin, David |
| المساهمون: | Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP), Université Grenoble Alpes (UGA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire CHU Grenoble (CHUGA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Etablissement français du sang - Auvergne-Rhône-Alpes (EFS) |
| المصدر: | ISSN: 1661-6596. |
| بيانات النشر: | CCSD MDPI |
| سنة النشر: | 2024 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | biodistribution, extracellular vesicles, live imaging, miRFP713, microbiota, outer membrane vesicles (OMVs), MESH: Animals, MESH: Mice, MESH: Humans, MESH: Escherichia coli, MESH: Tissue Distribution, MESH: Extracellular Vesicles, MESH: Intestines, MESH: Gram-Negative Bacteria, MESH: Bacterial Outer Membrane Proteins, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], [SDV.IB]Life Sciences [q-bio]/Bioengineering, [SDV.IMM]Life Sciences [q-bio]/Immunology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| الوصف: | International audience ; A cell’s ability to secrete extracellular vesicles (EVs) for communication is present in all three domains of life. Notably, Gram-negative bacteria produce a specific type of EVs called outer membrane vesicles (OMVs). We previously observed the presence of OMVs in human blood, which could represent a means of communication from the microbiota to the host. Here, in order to investigate the possible translocation of OMVs from the intestine to other organs, the mouse was used as an animal model after OMVs administration. To achieve this, we first optimized the signal of OMVs containing the fluorescent protein miRFP713 associated with the outer membrane anchoring peptide OmpA by adding biliverdin, a fluorescence cofactor, to the cultures. The miRFP713-expressing OMVs produced in E. coli REL606 strain were then characterized according to their diameter and protein composition. Native- and miRFP713-expressing OMVs were found to produce homogenous populations of vesicles. Finally, in vivo and ex vivo fluorescence imaging was used to monitor the distribution of miRFP713-OMVs in mice in various organs whether by intravenous injection or oral gavage. The relative stability of the fluorescence signals up to 3 days post-injection/gavage paves the way to future studies investigating the OMV-based communication established between the different microbiotas and their host. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38339099; PUBMED: 38339099; PUBMEDCENTRAL: PMC10855177 |
| DOI: | 10.3390/ijms25031821 |
| الاتاحة: | https://hal.science/hal-04467803 https://hal.science/hal-04467803v1/document https://hal.science/hal-04467803v1/file/Schaack%20et%20al.%20ijms.pdf https://doi.org/10.3390/ijms25031821 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2BEBB76 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms25031821 |
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