Academic Journal
The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene
| العنوان: | The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene |
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| المؤلفون: | Wimmer, Bernhard, Lottspeich, Friedrich, Klei, Ida van der, Veenhuis, Marten, Gietl, Christine |
| المصدر: | Wimmer , B , Lottspeich , F , Klei , I V D , Veenhuis , M & Gietl , C 1997 , ' The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 94 , no. 25 , pp. 13624-13629 . https://doi.org/10.1073/pnas.94.25.13624 |
| سنة النشر: | 1997 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | peroxisomes, microbodies, protein targeting, peroxisomal targeting signal 2, Citrullus vulgaris, MITOCHONDRIAL CARNITINE ACETYLTRANSFERASES, MALATE-DEHYDROGENASE, HANSENULA-POLYMORPHA, IMPORT, TRANSLOCATION, MEMBRANES, SEQUENCE, PRODUCTS, PATHWAY |
| الوصف: | The monoclonal a-70-kDa heat shock protein (hsp70) antibody recognizes in crude extracts from watermelon (Citrullus vulgaris) cotyledons with molecular masses of 70 and 72 KDa, Immunocytochemistry on watermelon cotyledon tissue and on isolated glyoxysomes identified hsp70s in the matrix of glyoxysomes and plastids. Affinity purification and partial amino acid determination revealed the 70-kDa protein to share high sequence identity with cytosolic hsp70s from a number of plant: species, while the 72 kDa protein was very similar to plastid hsp70s from pea and cucumber. A full-length cDNA clone encoding the 72-kDa hsp70 was isolated and identified two start methionines in frame within the N-terminal presequence leading either to an N-terminal extension of 67 amino acids or to a shorter one of 47 amino adds, The longer presequence was necessary and sufficient to target a reporter protein into watermelon proplastids in vitro, The shorter extension starting from the second methionine within the long version harbored a consensus peroxisomal targeting signal (RT-X-5-KL) that directed in vivo a reporter protein into peroxisomes of the yeast Hansenula polymorpha. Peroxisomal targeting was however prevented, when the 67-residue presequence was fused to the reporter protein, indicating that the peroxisomal targeting signal 2 information is hidden in this contest. We propose that the 72-kDa hsp70 is encoded by a single gene, bur targeted alternatively into two organelles by the modulated use of its presequence. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://research.rug.nl/en/publications/0551d66f-1982-4081-9eb8-4eefb32d2f6f |
| DOI: | 10.1073/pnas.94.25.13624 |
| الاتاحة: | https://hdl.handle.net/11370/0551d66f-1982-4081-9eb8-4eefb32d2f6f https://research.rug.nl/en/publications/0551d66f-1982-4081-9eb8-4eefb32d2f6f https://doi.org/10.1073/pnas.94.25.13624 https://pure.rug.nl/ws/files/3215362/1997ProcNatlAcadSciWimmer.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.2BE99497 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1073/pnas.94.25.13624 |
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