التفاصيل البيبلوغرافية
| العنوان: |
Model of Spot 42 sRNA-mediated generation of galET RNA species by Rho and RNase E. |
| المؤلفون: |
Heung Jin Jeon (9609293), Yonho Lee (9609302), Monford Paul Abishek N (9616067), Xun Wang (138298), Dhruba K. Chattoraj (9609305), Heon M. Lim (9609308) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Microbiology, Cell Biology, Genetics, Molecular Biology, Infectious Diseases, Computational Biology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, paired segments required, div >< p, rnase e together, endonuclease rnase e, transcript cleavage within, transcript cleavage requires, spot 42 srna, dependent transcription termination, potential srna targets, mrna base pairing, escherichia coli <, operon transcript leads, new mrna species, transcript cleavage, transcription termination, e <, mrna base, coli <, gene leads, two targets, galk < |
| الوصف: |
A) Generation of galET-short by Rho: The model shows the (leading) ribosome at the stop codon of galT on a nascent chain of gal mRNA at the galT-galK intercistronic region uncoupled from a transcribing RNA polymerase. In this situation, the mRNA sequences that pair with Spot 42 region II are not covered by the ribosome. B) The Region II binding is assumed to maintain downstream mRNA ribosome-free. This provides the platform for Rho loading and generation of galET-short by transcription termination by Rho at 2184. The 3’end of galET-short is underlined. C) The subsequent processing of the Rho-terminated transcript by RNase III cleavage is shown at the 3’end of galET-short (downward green arrow). D) Production of galET-long by RNase E: This process requires hybridization with Spot 42 Region I. We assume that translation termination at galT does not always lead to translation initiation of galK (due to stochastic uncoupling of galK translation from galT translation), which would keep galK RNA ribosome-free and promote hybridization with Region I. (E, F) The hybridization is assumed to ensure that the downstream RNA remains ribosome-free in the nascent transcript (E) or in non-nascent transcripts (F) to allow RNase E cleavage. The hybrid Region I could also block 3’-end processing by 3’→ 5’ exonucleases after RNase E cleavage. Note that Spot 42 Region III cannot base pair with the corresponding gal sequences in this situation due to the presence of the ribosome at galT stop codon, which might explain why mutations in that region are inconsequential to galET mRNA production. “RNase (?)” represents currently unidentified RNases(s) that may be involved in the further processing events of the galET transcripts. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Model_of_Spot_42_sRNA-mediated_generation_of_i_galET_i_RNA_species_by_Rho_and_RNase_E_/16899354 |
| DOI: |
10.1371/journal.pgen.1009878.g007 |
| الاتاحة: |
https://doi.org/10.1371/journal.pgen.1009878.g007 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.2BD796EF |
| قاعدة البيانات: |
BASE |