التفاصيل البيبلوغرافية
| العنوان: |
Cellular calcium oscillations in droplets with different chemical concentrations supplied by droplet-array sandwiching technology |
| المؤلفون: |
Konishi, Satoshi, Higuchi, Yuriko, Tamayori, Asuka |
| المساهمون: |
小西, 聡, 樋口, ゆり子, 玉寄, あすか |
| بيانات النشر: |
Elsevier BV |
| سنة النشر: |
2022 |
| المجموعة: |
Kyoto University Research Information Repository (KURENAI) / 京都大学学術情報リポジトリ |
| مصطلحات موضوعية: |
Microfluidics, HeLa cells, Electrowetting-on-dielectric (EWOD), Oscillation, Lab-on-a-chip, Wettability, Droplet-array sandwiching technology, Calcium oscillation, Histamine concentration |
| الوصف: |
DAST技術の独自展開によりバイオケミカル分野への幅広い応用を期待 --微量液滴間の接触によりヒスタミン供給量を調整し液滴内の細胞カルシウム振動の変化を初実証--. 京都大学プレスリリース. 2022-08-10. ; Digital microfluidics using droplets on a chip, such as droplet-array sandwiching technology, provide efficient tools for biochemistry from the perspective of time and sample consumption. Droplet-array sandwiching technology uses the fusion and separation of droplets on upper and lower substrates for high-throughput screening. In our previous work, we developed independent control of individual droplets for this technology using electrowetting-on-dielectric to control the droplet height and allow different chemical concentrations in droplets on the same chip. In this study, we explored the applicability of droplet-array sandwiching technology to cell-based analysis by observing cellular calcium oscillations in HeLa cells in droplets with different histamine concentrations. Histamine concentrations could be controlled by controlling contact time between upper and lower droplets. We found that calcium oscillations intensified with higher histamine concentrations prepared by controlling contact time. These results suggest that droplet-array sandwiching technology can be used for cell-based analysis, where the chemical concentration for cellular stimulation needs to be controlled. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
0925-4005 |
| Relation: |
https://www.kyoto-u.ac.jp/ja/research-news/2022-08-10-5; http://hdl.handle.net/2433/275865; Sensors and Actuators B: Chemical; 370; 132435 |
| الاتاحة: |
http://hdl.handle.net/2433/275865 |
| Rights: |
© 2022 The Authors. Published by Elsevier B.V. ; This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.2BD50126 |
| قاعدة البيانات: |
BASE |