Academic Journal
Depletion of the Third Complement Component Ameliorates Age‐Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model
| العنوان: | Depletion of the Third Complement Component Ameliorates Age‐Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model |
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| المؤلفون: | Rogińska, Dorota, Kawa, Miłosz P., Pius-Sadowska, Ewa, Lejkowska, Renata, Łuczkowska, Karolina, Wiszniewska, Barbara, Kaarniranta, Kai, Paterno, Jussi J., Schmidt, Christian A., Machaliński, Bogusław, Machalińska, Anna |
| المساهمون: | Ramana, Kota V., National Science Center |
| المصدر: | Oxidative Medicine and Cellular Longevity ; volume 2017, issue 1 ; ISSN 1942-0900 1942-0994 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2017 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | The aim of the study was to investigate the influence of complement component C3 global depletion on the biological structure and function of the aged retina. In vivo morphology (OCT), electrophysiological function (ERG), and the expression of selected oxidative stress‐, apoptosis‐, and autophagy‐related proteins were assessed in retinas of 12‐month‐old C3‐deficient and WT mice. Moreover, global gene expression in retinas was analyzed by RNA arrays. We found that the absence of active C3 was associated with (1) alleviation of the age‐dependent decrease in retinal thickness and gradual deterioration of retinal bioelectrical function, (2) significantly higher levels of antioxidant enzymes (catalase and glutathione reductase) and the antiapoptotic survivin and Mcl‐1/Bak dimer, (3) lower expression of the cellular oxidative stress marker—4HNE—and decreased activity of proapoptotic caspase‐3, (4) ameliorated retinal autophagic activity with localization of ubiquitinated protein conjugates commonly along the retinal pigment epithelium (RPE) layer, and (5) significantly increased expression of several gene sets associated with maintenance of the physiological functions of the neural retina. Our findings shed light on mechanisms of age‐related retinal alterations by identifying C3 as a potential therapeutic target for retinal aging. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1155/2017/5306790 |
| الاتاحة: | http://dx.doi.org/10.1155/2017/5306790 http://downloads.hindawi.com/journals/omcl/2017/5306790.pdf http://downloads.hindawi.com/journals/omcl/2017/5306790.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2017/5306790 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.2B6EEA39 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2017/5306790 |
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