Academic Journal
Simultaneous determination of polyamines, N-acetylated polyamines and the polyamine analogues BE-3-3-3 and BE-4-4-4-4 by capillary gas chromatography with nitrogen-phosphorus detection
| العنوان: | Simultaneous determination of polyamines, N-acetylated polyamines and the polyamine analogues BE-3-3-3 and BE-4-4-4-4 by capillary gas chromatography with nitrogen-phosphorus detection |
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| المؤلفون: | Dorhout, B, Kingma, AW, deHoog, E, Muskiet, FAJ |
| المصدر: | Dorhout , B , Kingma , AW , deHoog , E & Muskiet , FAJ 1997 , ' Simultaneous determination of polyamines, N-acetylated polyamines and the polyamine analogues BE-3-3-3 and BE-4-4-4-4 by capillary gas chromatography with nitrogen-phosphorus detection ' , Journal of Chromatography B , vol. 700 , no. 1-2 , pp. 23-30 . https://doi.org/10.1016/S0378-4347(97)00294-6 |
| سنة النشر: | 1997 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | polyamines, N-acetylpolyamines, N-1,N-11-bis(ethyl)norspermine, 1,19-bis(ethylamino)-5,10,15-triazanonadecane, putrescine, spermidine, spermine, GROWTH-INHIBITION, PHARMACOKINETICS, METABOLISM, INCREASES, DEPLETION, CELLS, URINE |
| الوصف: | We describe a method for the profiling of polyamines, N-acetylated polyamines and the polyamine analogues N-1,N-11 bis(ethyl)norspermine (BE-3-3-3) and 1,19-bis(ethylamino)-5,10,15-triazanonadecane (BE-4-4-4-4) in L1210 murine leukaemia cells by capillary gas chromatography with nitrogen-phosphorus detection. The method makes use of four internal standards. Prepurification comprises deproteinization, isolation with Sep-Pak silica at pH 9.0, conversion to heptafluorobutyryl derivatives and postderivatization organic fluid extraction. Within-and between-series precisions (given as C.V.s) for analysis of 1-2x10(6) cells were: putrescine 5.5 and 29.4%; spermidine 1.6 and 7.1%; and spermine 3.2 and 7.6%, respectively. Recoveries relative to the respective internal standards, were in the 70.6-104.7% range. Accuracy and precision of measurements of BE-4-4-4-4 can probably be improved by the introduction of a separate pentamine internal standard. We conclude that the method can be used for studying the effect of BE-3-3-3 and BE-4-4-4-4, and possibly their metabolites, on polyamine homeostasis (biosynthesis, retroconversion, transport, terminal catabolism) and polyamine function. (C) 1997 Elsevier Science B.V. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://research.rug.nl/en/publications/4e61344a-2664-43a8-b36b-5732f8fbd20f |
| DOI: | 10.1016/S0378-4347(97)00294-6 |
| الاتاحة: | https://hdl.handle.net/11370/4e61344a-2664-43a8-b36b-5732f8fbd20f https://research.rug.nl/en/publications/4e61344a-2664-43a8-b36b-5732f8fbd20f https://doi.org/10.1016/S0378-4347(97)00294-6 |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.2B041924 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/S0378-4347(97)00294-6 |
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