Academic Journal
MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function
| العنوان: | MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function |
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| المؤلفون: | Hume, L, Edge, JC, Tinning, H, Wang, D, Taylor, AS, Ovchinnikov, V, Geijer-Simpson, A, Vrljicak, P, Brosens, JJ, Lucas, ES, Simpson, NAB, Shillito, J, Forbes, K, O’Connell, MJ, Forde, N |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2023 |
| المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| الوصف: | We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 mg/ml) for 24 h coordinate with 1,713 of their predicted targets. Proteomic analysis of cells transfected with miRNA mimic/inhibitor (48 h: n = 3) revealed 1,745 proteins altered by miR-340-5p (mimic; 1,369, inhibitor; 376) of which 171 were predicted targets and P4-regulated. MiR-542-3p altered 2,353 (mimic; 1,378, inhibitor; 975) 100 which were mirDB predicted, including 46 P4-regulated. MiR-671-5p altered 1,744 proteins (mimic; 1,252, inhibitor; 492) 95 of which were predicted targets and 46 P4-regulated. All miRNAs were detected in luteal phase endometrial biopsies, irrespective of pregnancy outcomes. miR-340-5p expression increased in biopsies from individuals suffering previous and subsequent miscarriage compared to those with subsequent live birth. Dysfunction of these miRNAs and their targets contribute to endometrial-derived recurrent pregnancy loss. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://eprints.whiterose.ac.uk/197107/1/MicroRNAs%20emerging%20coordinate%20with%20placental.pdf; Hume, L, Edge, JC, Tinning, H orcid.org/0000-0002-0840-3205 et al. (12 more authors) (2023) MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function. iScience, 26 (4). 106339. ISSN 2589-0042 |
| DOI: | 10.1016/j.isci.2023.106339 |
| الاتاحة: | https://eprints.whiterose.ac.uk/197107/ https://eprints.whiterose.ac.uk/197107/1/MicroRNAs%20emerging%20coordinate%20with%20placental.pdf https://doi.org/10.1016/j.isci.2023.106339 |
| Rights: | cc_by_4 |
| رقم الانضمام: | edsbas.2B0337B0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.isci.2023.106339 |
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