التفاصيل البيبلوغرافية
| العنوان: |
Adapting a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Gas-Phase Fluorescence Spectroscopy Measurement of Trapped Biomolecular Ions |
| المؤلفون: |
Ri Wu (2747599), Jonas B. Metternich (9689041), Prince Tiwari (1356759), Renato Zenobi (248557) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Microbiology, Neuroscience, Pharmacology, Biotechnology, Developmental Biology, Infectious Diseases, Space Science, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, quadrupole ion traps, lens installed beside, cylindrical icr cell, back trapping plate, art optical system, allowing ∼ 10, phase fluorescence spectroscopy, perform fluorescence experiments, fluorescence lifetime measurement, fluorescence experiments could, various optical properties, tunable femtosecond laser, 97 ± 0, wire mesh replaced, e ., peptides, broad mass range, collected fluorescence light |
| الوصف: |
Gas-phase fluorescence spectroscopy is still in its infancy, which demands further instrumental developments. In this study, a Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS), equipped with a lab-developed data acquisition system, was coupled to a tunable femtosecond laser and a state-of-the-art optical system for fluorescence studies of mass-selected ions. For excitation, a laser beam was focused (beam size < 1.0 mm) into the cylindrical ICR cell. A wire mesh replaced the back trapping plate, allowing ∼10% of the fluorescence emitted from trapped ions to be collected by a lens installed beside the wire mesh. The collected fluorescence light was then transmitted outside of the mass spectrometer via fiber optics. A novel accumulation during detection (ADD) scheme was developed to increase the duty cycle of gas-phase fluorescence spectroscopy experiments. With ADD, >90% duty cycle for mass spectrometry and fluorescence experiments could be achieved. This instrument was able to perform fluorescence experiments on various ions, from simple rhodamine dyes to large biomolecules (i.e., peptides and proteins) labeled with dyes of various optical properties. A fluorescence lifetime measurement of trapped rhodamine 6G cations was also performed, yielding a value of 5.97 ± 0.23 ns. This setup has a broad mass range and decent fluorescence spectroscopy performance (i.e., the emission spectrum of rhodamine 6G can be acquired with good S / N in a minute). Finally, this setup also allows more challenging gas-phase fluorescence spectroscopy experiments, for example, of low quantum yield fluorophores and large biomolecules in their native state that appear at high m/z , which may not be doable with quadrupole ion traps (QIT). |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Adapting_a_Fourier_Transform_Ion_Cyclotron_Resonance_Mass_Spectrometer_for_Gas-Phase_Fluorescence_Spectroscopy_Measurement_of_Trapped_Biomolecular_Ions/17029525 |
| DOI: |
10.1021/acs.analchem.1c02858.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.1c02858.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.2AE8F4C4 |
| قاعدة البيانات: |
BASE |