Academic Journal
Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Molecular Typing of Acinetobacter baumannii in Comparison with Orthogonal Methods
| العنوان: | Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Molecular Typing of Acinetobacter baumannii in Comparison with Orthogonal Methods |
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| المؤلفون: | Busby, Eloise J, Doyle, Ronan M, Leboreiro Babe, Clara, Harris, Kathryn A, Mack, Damien, Méndez-Cervantes, Gema, O'Sullivan, Denise M, Pang, Vicky, Sadouki, Zahra, Solanki, Priya, Huggett, Jim F, McHugh, Timothy D, Wey, Emmanuel Q |
| المصدر: | Microbiol Spectrum , Article e0499522. (2023) (In press). |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 2023 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Acinetobacter, MALDI-TOF MS, molecular subtyping |
| الوصف: | Colonization and subsequent health care-associated infection (HCAI) with Acinetobacter baumannii are a concern for vulnerable patient groups within the hospital setting. Outbreaks involving multidrug-resistant strains are associated with increased patient morbidity and mortality and poorer overall outcomes. Reliable molecular typing methods can help to trace transmission routes and manage outbreaks. In addition to methods deployed by reference laboratories, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) may assist by making initial in-house judgments on strain relatedness. However, limited studies on method reproducibility exist for this application. We applied MALDI-TOF MS typing to A. baumannii isolates associated with a nosocomial outbreak and evaluated different methods for data analysis. In addition, we compared MALDI-TOF MS with whole-genome sequencing (WGS) and Fourier transform infrared spectroscopy (FTIR) as orthogonal methods to further explore their resolution for bacterial strain typing. A related subgroup of isolates consistently clustered separately from the main outbreak group by all investigated methods. This finding, combined with epidemiological data from the outbreak, indicates that these methods identified a separate transmission event unrelated to the main outbreak. However, the MALDI-TOF MS upstream approach introduced measurement variability impacting method reproducibility and limiting its reliability as a standalone typing method. Availability of in-house typing methods with well-characterized sources of measurement uncertainty could assist with rapid and dependable confirmation (or denial) of suspected transmission events. This work highlights some of the steps to be improved before such tools can be fully integrated into routine diagnostic service workflows for strain typing. IMPORTANCE Managing the transmission of antimicrobial resistance necessitates reliable methods for tracking outbreaks. We compared the performance of MALDI-TOF MS with ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://discovery.ucl.ac.uk/id/eprint/10169774/1/spectrum.04995-22.pdf; https://discovery.ucl.ac.uk/id/eprint/10169774/ |
| الاتاحة: | https://discovery.ucl.ac.uk/id/eprint/10169774/1/spectrum.04995-22.pdf https://discovery.ucl.ac.uk/id/eprint/10169774/ |
| Rights: | open |
| رقم الانضمام: | edsbas.2AAA9A7F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |