Dissertation/ Thesis
Use of peptidic scaffolds for the control of the self-assembly of Guanine derivatives: From guanines tetrads to nucleic acids quadruplexes. ; Utilisation de gabarits peptidiques pour le contrôle de l'auto-assemblage de dérivés de la Guanine : Des tétrades de guanines aux quadruplexes d'acides nucléiques.
| العنوان: | Use of peptidic scaffolds for the control of the self-assembly of Guanine derivatives: From guanines tetrads to nucleic acids quadruplexes. ; Utilisation de gabarits peptidiques pour le contrôle de l'auto-assemblage de dérivés de la Guanine : Des tétrades de guanines aux quadruplexes d'acides nucléiques. |
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| المؤلفون: | Murat, Pierre |
| المساهمون: | Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Grenoble, Eric Defrancq(eric.defrancq@ujf-grenoble.fr) |
| المصدر: | https://theses.hal.science/tel-00540497 ; Chimie. Université de Grenoble, 2010. Français. ⟨NNT : ⟩. |
| بيانات النشر: | CCSD |
| سنة النشر: | 2010 |
| المجموعة: | Université Grenoble Alpes: HAL |
| مصطلحات موضوعية: | Guanines tetrads, DNA quadruplexes, Quadruplexes Mimics, Supramolecular chemistry, Cyclodecapeptidic scaffold, Chemioselective ligations, Biosensors, Tétrades de guanines, Quadruplexes d'ADN, Mimes de quadruplexes, Chimie supramoléculaire, Châssis cyclodécapeptidique cyclique, Ligations chimiosélectives, SPR, Biosenseurs, [CHIM]Chemical Sciences |
| الوصف: | It is known for almost 50 years, that guanosine derivatives can form tetrads under specific conditions. Guanine tetrads, which are formed by the coplanar arrangement of four guanines through the formation of Hoogsteen hydrogen bonds, represent a relevant model for the study of molecular self-assembly processes and non covalent synthesis. In the first part of this manuscript, we will describe how a peptidic scaffold can be used to constrain the formation of a tetrad. The introduction of conformational constraints allows the control of the formation of synthetic guanines tetrads in water in the absence of cations to stabilize the structure. Two architectures have been synthesized and characterized and the results highlight the different phenomena that allow the formation of synthetic tetrads. It is also known that guanine rich nucleic acids sequences can form particular secondary structures, known as G-quadruplexes. These motifs represent relevant target for the development of new anticancer therapies since they are involved in a lot of biological events (telomere maintenance, oncogene activation .). However the study of the interaction between small organic molecules and such motifs are difficult due to their high polymorphism. We propose in a second chapter the design and the synthesis of G-quadruplexes mimics based on the covalent attachment of short nucleic acids sequences on a peptidic scaffold, which allow the control of the topologies of the so-obtained quadruplexes. The study of the interactions of small organic molecules with these mimics has been performed by SPR studies. This approach appears to be very interesting to understand the interactions that are involved in the recognition of quadruplexes and the selection of new G4 ligands. ; Il est connu depuis presque 50 ans que, dans certaines conditions, les dérivés de guanosine peuvent former des tétrades. Ces tétrades résultent de l'arrangement coplanaire de quatre guanines reliées par des liaisons hydrogène de type Hoogsteen. Ces motifs particuliers ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | French |
| الاتاحة: | https://theses.hal.science/tel-00540497 https://theses.hal.science/tel-00540497v1/document https://theses.hal.science/tel-00540497v1/file/ThA_se_PMurat.pdf |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.2A6D06F5 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |