Academic Journal
Structure of the multi-subunit chloroplast RNA polymerase
| العنوان: | Structure of the multi-subunit chloroplast RNA polymerase |
|---|---|
| المؤلفون: | do Prado, P., Ahrens, F., Liebers, M., Ditz, N., Braun, H., Pfannschmidt, T., Hillen, H. |
| المصدر: | Molecular Cell |
| سنة النشر: | 2024 |
| المجموعة: | Max Planck Society: MPG.PuRe |
| الوصف: | Chloroplasts contain a dedicated genome that encodes subunits of the photosynthesis machinery. Transcription of photosynthesis genes is predominantly carried out by a plastid-encoded RNA polymerase (PEP), a nearly 1 MDa complex composed of core subunits with homology to eubacterial RNA polymerases (RNAPs) and at least 12 additional chloroplast-specific PEP-associated proteins (PAPs). However, the architecture of this complex and the functions of the PAPs remain unknown. Here, we report the cryo-EM structure of a 19-subunit PEP complex from Sinapis alba (white mustard). The structure reveals that the PEP core resembles prokaryotic and nuclear RNAPs but contains chloroplast-specific features that mediate interactions with the PAPs. The PAPs are unrelated to known transcription factors and arrange around the core in a unique fashion. Their structures suggest potential functions during transcription in the chemical environment of chloroplasts. These results reveal structural insights into chloroplast transcription and provide a framework for understanding photosynthesis gene expression. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | info:eu-repo/grantAgreement/EC/H2020/101116869; http://hdl.handle.net/21.11116/0000-000F-0E37-5; http://hdl.handle.net/21.11116/0000-000F-0E39-3 |
| الاتاحة: | http://hdl.handle.net/21.11116/0000-000F-0E37-5 http://hdl.handle.net/21.11116/0000-000F-0E39-3 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.2A14E53B |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |