Prolonged DNA hydrolysis in water: A study on DNA stability

التفاصيل البيبلوغرافية
العنوان:	Prolonged DNA hydrolysis in water: A study on DNA stability
المؤلفون:	Fattorini P, Marrubini G, Bonin S, Bertoglio B, Grignani P, Recchia E, Pitacco P, Procopio F, Cantoni C, Pajnič IZ, Sorçaburu-Cigliero S, Previdere C.
المساهمون:	Fattorini, P, Marrubini, G, Bonin, S, Bertoglio, B, Grignani, P, Recchia, E, Pitacco, P, Procopio, F, Cantoni, C, Pajnič, Iz, Sorçaburu-Cigliero, S, Previdere, C.
سنة النشر:	2018
المجموعة:	Università degli studi di Trieste: ArTS (Archivio della ricerca di Trieste)
مصطلحات موضوعية:	DNA hydrolysi, DNA quantification, Degraded sample, qPCR
الوصف:	This work provides a protocol for the in vitro production of damaged DNA samples. In particular, heat-mediated hydrolysis of the samples at 70 °C in ultrapure water was performed in 1.7 mL Eppendorf tubes sealed by Parafilm for 0-36 h. The chemical/physical features of the resulting samples are described. After normalization of the qPCR data, these were compared with those obtained from samples treated for 0-10 h in a previous study.
نوع الوثيقة:	article in journal/newspaper
وصف الملف:	ELETTRONICO
اللغة:	English
ردمك:	978-2-35234-091-1 2-35234-091-8
Relation:	info:eu-repo/semantics/altIdentifier/pmid/30238033; info:eu-repo/semantics/altIdentifier/wos/WOS:000450242200185; volume:20; firstpage:1237; lastpage:1243; numberofpages:7; journal:DATA IN BRIEF; http://hdl.handle.net/11368/2929794; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85053389487; https://www.sciencedirect.com/science/article/pii/S2352340918309727?via%3Dihub
DOI:	10.1016/j.dib.2018.08.120
الاتاحة:	http://hdl.handle.net/11368/2929794 https://doi.org/10.1016/j.dib.2018.08.120 https://www.sciencedirect.com/science/article/pii/S2352340918309727?via%3Dihub
Rights:	info:eu-repo/semantics/openAccess
رقم الانضمام:	edsbas.2A087FC9
قاعدة البيانات:	BASE

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الوصف
ردمك:	9782352340911 2352340918
DOI:	10.1016/j.dib.2018.08.120