Academic Journal
Insertional mutagenesis in ChordinA induced by endogenous ΔTgf2 transposon leads to bifurcation of axial skeletal systems in grass goldfish
| العنوان: | Insertional mutagenesis in ChordinA induced by endogenous ΔTgf2 transposon leads to bifurcation of axial skeletal systems in grass goldfish |
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| المؤلفون: | Guo, Dan-Dan, Sun, Yi-Wen, Cui, Wen-Tao, Guo, Hong-Hong, Du, Shang-Ke, Chen, Jie, Zou, Shu-Ming |
| المصدر: | Scientific Reports ; volume 9, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2019 |
| الوصف: | The grass goldfish appeared early in the evolutionary history of goldfish, and shows heritable stability in the development of the caudal fin. The twin-tail phenotype is extremely rare, however, some twin-tail individuals were produced in the process of breeding for ornamental value. From mutations in the twin-tail goldfish genome, we identified two kinds of Tgf2 transposons. One type was completely sequenced Tgf2 and the other type was ΔTgf2 , which had 858 bp missing. We speculate that the bifurcation of the axial skeletal system in goldfish may be caused by an endogenous ΔTgf2 insertion mutation in Chordin A , as ΔTgf2 has no transposition activity and blocks the expression of Chordin A . The twin-tail showed doubled caudal fin and accumulation of red blood cells in the tail. In addition, in situ hybridization revealed that ventral embryonic tissue markers ( eve1 , sizzled , and bmp4 ) were more widely and strongly expressed in the twin-tail than in the wild-type embryos during the gastrula stage, and bmp4 showed bifurcated expression patterns in the posterior region of the twin-tail embryos. These results provide new insights into the artificial breeding of genetically stable twin-tail grass goldfish families. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-019-40651-1 |
| الاتاحة: | http://dx.doi.org/10.1038/s41598-019-40651-1 https://www.nature.com/articles/s41598-019-40651-1.pdf https://www.nature.com/articles/s41598-019-40651-1 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.29E9247 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-019-40651-1 |
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