Academic Journal
H2S-Synthesizing Enzymes Are Putative Determinants in Lung Cancer Management toward Personalized Medicine
| العنوان: | H2S-Synthesizing Enzymes Are Putative Determinants in Lung Cancer Management toward Personalized Medicine |
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| المؤلفون: | Hipólito, Ana, Mendes, Cindy, Martins, Filipa, Lemos, Isabel, Francisco, Inês, Cunha, Fernando, Almodôvar, Teresa, Albuquerque, Cristina, Gonçalves, Luís G, Bonifácio, Vasco D B, Vicente, João B, Serpa, Jacinta |
| المساهمون: | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), iNOVA4Health - pólo NMS, Molecular, Structural and Cellular Microbiology (MOSTMICRO), Instituto de Tecnologia Química e Biológica António Xavier (ITQB) |
| سنة النشر: | 2024 |
| المجموعة: | Repositório da Universidade Nova de Lisboa (UNL) |
| مصطلحات موضوعية: | 3-mercaptopyruvate sulfurtransferase (MST), NSCLC metabolism, cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), cysteine dioxygenase (CDO1), cysteine metabolism, metabolic plasticity, metabolism-based therapies, SDG 3 - Good Health and Well-being |
| الوصف: | Lung cancer is a lethal disease with no truly efficient therapeutic management despite the progresses, and metabolic profiling can be a way of stratifying patients who may benefit from new therapies. The present study is dedicated to profiling cysteine metabolic pathways in NSCLC cell lines and tumor samples. This was carried out by analyzing hydrogen sulfide (H2S) and ATP levels, examining mRNA and protein expression patterns of cysteine catabolic enzymes and transporters, and conducting metabolomics analysis using nuclear magnetic resonance (NMR) spectroscopy. Selenium-chrysin (SeChry) was tested as a therapeutic alternative with the aim of having an effect on cysteine catabolism and showed promising results. NSCLC cell lines presented different cysteine metabolic patterns, with A549 and H292 presenting a higher reliance on cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) to maintain H2S levels, while the PC-9 cell line presented an adaptive behavior based on the use of mercaptopyruvate sulfurtransferase (MST) and cysteine dioxygenase (CDO1), both contributing to the role of cysteine as a pyruvate source. The analyses of human lung tumor samples corroborated this variability in profiles, meaning that the expression of certain genes may be informative in defining prognosis and new targets. Heterogeneity points out individual profiles, and the identification of new targets among metabolic players is a step forward in cancer management toward personalized medicine. ; publishersversion ; published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2076-3921 |
| Relation: | PURE: 82240754; PURE UUID: 5a39e0e7-c874-4648-aa6d-65fbc656119e; PubMed: 38247476; ORCID: /0000-0001-5683-3552/work/151887282; ORCID: /0000-0002-1548-5907/work/151887775; Scopus: 85183131250; http://hdl.handle.net/10362/162860; https://doi.org/10.3390/antiox13010051 |
| DOI: | 10.3390/antiox13010051 |
| الاتاحة: | http://hdl.handle.net/10362/162860 https://doi.org/10.3390/antiox13010051 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.29D29925 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20763921 |
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| DOI: | 10.3390/antiox13010051 |