التفاصيل البيبلوغرافية
| العنوان: |
The Impact of Dose Rate on DNA Double-Strand Break Formation and Repair in Human Lymphocytes Exposed to Fast Neutron Irradiation |
| المؤلفون: |
Shankari Nair, Monique Engelbrecht, Xanthene Miles, Roya Ndimba, Randall Fisher, Peter du Plessis, Julie Bolcaen, Jaime Nieto-Camero, Evan de Kock, Charlot Vandevoorde |
| المصدر: |
International Journal of Molecular Sciences; Volume 20; Issue 21; Pages: 5350 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2019 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
DNA double-strand breaks, γ-H2AX, neutrons, low dose rate, high dose rate, lymphocytes |
| جغرافية الموضوع: |
agris |
| الوصف: |
The lack of information on how biological systems respond to low-dose and low dose-rate exposures makes it difficult to accurately assess the carcinogenic risks. This is of critical importance to space radiation, which remains a serious concern for long-term manned space exploration. In this study, the γ-H2AX foci assay was used to follow DNA double-strand break (DSB) induction and repair following exposure to neutron irradiation, which is produced as secondary radiation in the space environment. Human lymphocytes were exposed to high dose-rate (HDR: 0.400 Gy/min) and low dose-rate (LDR: 0.015 Gy/min) p(66)/Be(40) neutrons. DNA DSB induction was investigated 30 min post exposure to neutron doses ranging from 0.125 to 2 Gy. Repair kinetics was studied at different time points after a 1 Gy neutron dose. Our results indicated that γ-H2AX foci formation was 40% higher at HDR exposure compared to LDR exposure. The maximum γ-H2AX foci levels decreased gradually to 1.65 ± 0.64 foci/cell (LDR) and 1.29 ± 0.45 (HDR) at 24 h postirradiation, remaining significantly higher than background levels. This illustrates a significant effect of dose rate on neutron-induced DNA damage. While no significant difference was observed in residual DNA damage after 24 h, the DSB repair half-life of LDR exposure was slower than that of HDR exposure. The results give a first indication that the dose rate should be taken into account for cancer risk estimations related to neutrons. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Biology; https://dx.doi.org/10.3390/ijms20215350 |
| DOI: |
10.3390/ijms20215350 |
| الاتاحة: |
https://doi.org/10.3390/ijms20215350 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.29AB1132 |
| قاعدة البيانات: |
BASE |