Academic Journal
REACTION OF BLOOD TRANSFORMATION OF BLOOD LYMPHOCYTES OF NONLINEAR FEMALES RATS, THEIR OFFSPRING АT THE EARLY STAGES OF POST-EMBRYONIC DEVELOPMENT ON THE BASIS OF THE INFLUENCE OF SALINE EXTRACT HIRUDO VERBANA ; РЕАКЦІЯ БЛАСТНОЇ ТРАНСФОРМАЦІЇ ЛІМФОЦИТІВ КРОВІ НЕЛІНІЙНИХ САМИЦЬ ЩУРІВ, ЇХ ПРИПЛОДУ НА РАННІХ ЕТАПАХ ПОСТЕМБРІОНАЛЬНОГО РОЗВИТКУ НА ФОНІ ВПЛИВУ СОЛЬОВОГО ЕКСТРАКТУ HIRUDO VERBANA
| العنوان: | REACTION OF BLOOD TRANSFORMATION OF BLOOD LYMPHOCYTES OF NONLINEAR FEMALES RATS, THEIR OFFSPRING АT THE EARLY STAGES OF POST-EMBRYONIC DEVELOPMENT ON THE BASIS OF THE INFLUENCE OF SALINE EXTRACT HIRUDO VERBANA ; РЕАКЦІЯ БЛАСТНОЇ ТРАНСФОРМАЦІЇ ЛІМФОЦИТІВ КРОВІ НЕЛІНІЙНИХ САМИЦЬ ЩУРІВ, ЇХ ПРИПЛОДУ НА РАННІХ ЕТАПАХ ПОСТЕМБРІОНАЛЬНОГО РОЗВИТКУ НА ФОНІ ВПЛИВУ СОЛЬОВОГО ЕКСТРАКТУ HIRUDO VERBANA |
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| المؤلفون: | Амінов, Р. Ф., Фролов, О. К., Федотов, Є. Р. |
| المصدر: | Acta Biologica Ukrainica; No. 1 (2018): Bulletin of Zaporizhzhia National University. Biological Sciences; 46-52 ; Acta Biologica Ukrainica; № 1 (2018): Вісник Запорізького національного університету. Біологічні науки; 46-52 ; 2410-0943 |
| بيانات النشر: | Zaporizhzhia National University |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | hirudology, antigens, biologically active substances, гірудологія, антигени, біологічно активні речовини |
| الوصف: | Hirudotherapy is one of the methods of non-medical, and natural effects on the body. Therapeutic effects of HT due to the composition of the secret salivary glands of medical leeches are more than 100 biologically active substances. Such multicomponent secretion of salivary glands of leech reveals anticosmic, lipolytic, analgesic, immunomodulatory, anticoagulant, thrombolytic, anti-inflammatory and bactericidal action. Therefore, it was important to investigate the reaction of blast transformation of whole blood lymphocytes against the background of the influence of saline extracts from the organs of medical leeches. Female non-linear rats once a week, 2 weeks before the conjugate with males and 2 weeks afterwards, intraperitoneal antigens of the medicinal leech extract Hirudo verbana (Carena, 1820) were obtained by the method proposed by Frolov (2013). Dosage of antigens of the salt extract was carried out on the protein content (determined by Lowry). Fixed animals using a fixing device. Animals were divided into two groups: the first experimental group of animals under the influence of antigens of the salt extract of medical leeches at a dose of 1/10 of LD50 (at the rate of 5 mg / kg of animal weight), 0,5 ml; The second control group of animals, which were intraperitoneally injected with a 0,5 ml saline solution. The females were studied after feeding the offspring and breeding in dynamics on 45 th and 60 th day. All animals were decapitated under ethereal anesthesia. The proliferative activity of blood lymphocytes was evaluated in the whole blood by the reaction of blast transfection of lymphocytes (RBTL). Blood dissolved in heparin 350 μl and bring to 2,5 ml nutrient medium (1 : 5). The mixture was poured into penicillin vials and saturated with CO2 pipettes. The culture mixture was introduced by mitogen – konkanavalin A (KonA, Germany) at a dose of 10 μg / ml. As antigens (AG), salt extract was used from bodies N. verbana Carena, 1820 at a dose of 5 μg / ml. The concentration of protein in the extract was ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://journalsofznu.zp.ua/index.php/biology/article/view/6/18; http://journalsofznu.zp.ua/index.php/biology/article/view/6 |
| DOI: | 10.26661/2410-0943-2018-1-06 |
| الاتاحة: | http://journalsofznu.zp.ua/index.php/biology/article/view/6 https://doi.org/10.26661/2410-0943-2018-1-06 |
| رقم الانضمام: | edsbas.29670DEE |
| قاعدة البيانات: | BASE |
| DOI: | 10.26661/2410-0943-2018-1-06 |
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