Academic Journal
Concentrations of Insulin Glargine and Its Metabolites During Long-Term Insulin Therapy in Type 2 Diabetic Patients and Comparison of Effects of Insulin Glargine, Its Metabolites, IGF-I, and Human Insulin on Insulin and IGF-I Receptor Signaling
| العنوان: | Concentrations of Insulin Glargine and Its Metabolites During Long-Term Insulin Therapy in Type 2 Diabetic Patients and Comparison of Effects of Insulin Glargine, Its Metabolites, IGF-I, and Human Insulin on Insulin and IGF-I Receptor Signaling |
|---|---|
| المؤلفون: | Varewijck, Aimee, Yki-Jarvinen, H, Schmidt, R, Tennagels, N, Janssen, J.A.M.J.L. |
| المصدر: | Varewijck , A , Yki-Jarvinen , H , Schmidt , R , Tennagels , N & Janssen , J A M J L 2013 , ' Concentrations of Insulin Glargine and Its Metabolites During Long-Term Insulin Therapy in Type 2 Diabetic Patients and Comparison of Effects of Insulin Glargine, Its Metabolites, IGF-I, and Human Insulin on Insulin and IGF-I Receptor Signaling ' , Diabetes , vol. 62 , no. 7 , pp. 2539-2544 . https://doi.org/10.2337/db12-1773 |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | /dk/atira/pure/keywords/researchprograms/AFL001000/EMCMM013901, name=EMC MM-01-39-01, /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being, name=SDG 3 - Good Health and Well-being |
| الوصف: | We investigated 1) the ability of purified glargine (GLA), metabolites 1 (M1) and 2 (M2), IGF-I, and NPH insulin to activate the insulin receptor (IR)-A and IR-B and IGF-I receptor (IGF-IR) in vitro; 2) plasma concentrations of GLA, M1, and M2 during long-term insulin therapy in type 2 diabetic patients; and 3) IR-A and IR-B activation in vitro induced by serum from patients treated with GLA or NPH insulin. A total of 104 patients (age 56.3 +/- 0.8 years, BMI 31.4 +/- 0.5 kg/m(2), and A1C 9.1 +/- 0.1% [mean +/- SE]) were randomized to GLA or NPH insulin therapy for 36 weeks. Plasma concentrations of GLA, M1, and M2 were determined by liquid chromatography-tandem mass spectrometry assay. IR-A, IR-B, and IGF-IR autophosphorylation was induced by purified hormones or serum by kinase receptor activation assays. In vitro, M1 induced comparable IR-A, IR-B, and IGF-IR autophosphorylation (activation) as NPH insulin. After 36 weeks, M1 increased from undetectable (<0.2 ng/mL) to 1.5 ng/mL (0.9-2.1), while GLA and M2 remained undetectable. GLA dose correlated with M1 (r = 0.84; P < 0.001). Serum from patients treated with GLA or NPH insulin induced similar IR-A and IR-B activation. These data suggest that M1 rather than GLA mediates GLA effects and that compared with NPH insulin, GLA does not increase IGF-IR signaling during long-term insulin therapy in type 2 diabetes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| DOI: | 10.2337/db12-1773 |
| الاتاحة: | https://pure.eur.nl/en/publications/5cfe228e-4ffe-4b69-80ac-2b7336e2e9d5 https://doi.org/10.2337/db12-1773 https://pure.eur.nl/ws/files/47522578/REPUB_63963_OA.pdf http://hdl.handle.net/1765/63963 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.293AFA37 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2337/db12-1773 |
|---|