Academic Journal
Imaging inflammation in atherosclerotic plaques, targeting SST2 with [111In]In-DOTA-JR11
| العنوان: | Imaging inflammation in atherosclerotic plaques, targeting SST2 with [111In]In-DOTA-JR11 |
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| المؤلفون: | Meester, E.J. (Eric Jan), Krenning, B.J. (Boudewijn), Blois, E. (Erik) de, de Jong, M. (Marion), Steen, A.F.W. (Ton) van der, Bernsen, M.R. (Monique), Heiden, K. (Kim) van der |
| المصدر: | Journal of Nuclear Cardiology |
| سنة النشر: | 2020 |
| المجموعة: | RePub - Publications from Erasmus University, Rotterdam |
| مصطلحات موضوعية: | atherosclerosis, inflammation, molecular imaging, SPECT |
| الوصف: | Background: Imaging Somatostatin Subtype Receptor 2 (SST2) expressing macrophages by [DOTA,Tyr3]-octreotate (DOTATATE) has proven successful for plaque detection. DOTA-JR11 is a SST2 targeting ligand with a five times higher tumor uptake than DOTATATE, and holds promise to improve plaque imaging. The aim of this study was to evaluate the potential of DOTA-JR11 for plaque detection. Methods and Results: Atherosclerotic ApoE−/− mice (n = 22) fed an atherogenic diet were imaged by SPECT/CT two hours post injection of [111In]In-DOTA-JR11 (~ 200 pmol, ~ 50 MBq). In vivo plaque uptake of [111In]In-DOTA-JR11 was visible in all mice, with a target-to-background-ratio (TBR) of 2.23 ± 0.35. Post-mortem scans after thymectomy and ex vivo scans of the arteries after excision of the arteries confirmed plaque uptake of the radioligand with TBRs of 2.46 ± 0.52 and 3.43 ± 1.45 respectively. Oil red O lipid-staining and ex vivo autoradiography of excised arteries showed [111In]In-DOTA-JR11 uptake at plaque locations. Histological processing showed CD68 (macrophages) and SST2 expressing cells in plaques. SPECT/CT, in vitro autoradiography and immunohistochemistry performed on slices of a human carotid endarterectomy sample showed [111In]In-DOTA-JR11 uptake at plaque locations containing CD68 and SST2 expressing cells. Conclusions: The results of this study indicate DOTA-JR11 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://repub.eur.nl/pub/124713; urn:hdl:1765/124713 |
| DOI: | 10.1007/s12350-020-02046-y |
| الاتاحة: | http://repub.eur.nl/pub/124713 https://doi.org/10.1007/s12350-020-02046-y |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.2894194C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s12350-020-02046-y |
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