Academic Journal
The Proteolipid Protein Promoter Drives Expression outside of the Oligodendrocyte Lineage during Embryonic and Early Postnatal Development
| العنوان: | The Proteolipid Protein Promoter Drives Expression outside of the Oligodendrocyte Lineage during Embryonic and Early Postnatal Development |
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| المؤلفون: | Michalski, John-Paul |
| سنة النشر: | 2011 |
| المجموعة: | University of Ottawa: uO Research |
| مصطلحات موضوعية: | proteolipid protein (Plp), oligodendrocytes, myelin, central nervous system (CNS), embryonic development, early postnatal development |
| الوصف: | The proteolipid protein (Plp) gene promoter is responsible for driving expression of one of the major components of myelin – PLP and its splice variant DM-20. Both products are classically thought to express predominantly in oligodendrocytes. However, accumulating evidence suggests Plp expression is more widespread than previously thought. In an attempt to create a mouse model for inducing oligodendrocyte-specific gene deletions, we have generated transgenic mice expressing a Cre recombinase cDNA under control of the mouse Plp promoter. We demonstrate Plp promoter driven Cre expression is restricted predominantly to mature oligodendrocytes of the central nervous system (CNS) at postnatal day 28. However, crosses into the Rosa26LacZ and mT/mG reporter mouse lines reveal robust and widespread Cre activity in neuronal tissues at E15.5 and E10.5 that is not strictly oligodendrocyte lineage specific. By P28, all CNS tissues examined displayed high levels of reporter gene expression well outside of defined white matter zones. Importantly, our study reinforces the emerging idea that Plp promoter activity is not restricted to the myelinating cell lineage, but rather, has widespread activity both during embryonic and early postnatal development in the CNS. Specificity of the promoter to the oligodendrocyte cell lineage, as shown through the use of a tamoxifen inducible Plp-CreERt line, occurs only at later postnatal stages. Understanding the temporal shift in Plp driven expression is of consequence when designing experimental models to study oligodendrocyte biology. ; This work was supported by a grant from the Multiple Sclerosis Society of Canada to R.K. J-P.M. is supported by a Studentship from the Multiple Sclerosis Society of Canada. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This work was also supported by a grant from the University of Ottawa Open Access Author Fund. The funders had no role in study design, data collection and ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://hdl.handle.net/10393/20476; http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019772 |
| DOI: | 10.1371/journal.pone.0019772 |
| الاتاحة: | http://hdl.handle.net/10393/20476 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019772 https://doi.org/10.1371/journal.pone.0019772 |
| رقم الانضمام: | edsbas.28608335 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pone.0019772 |
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