التفاصيل البيبلوغرافية
| العنوان: |
Antibacterial Mechanism of Gloverin2 from Silkworm, Bombyx mori |
| المؤلفون: |
Qian Wang, Pengchao Guo, Zhan Wang, Huawei Liu, Yunshi Zhang, Shan Jiang, Wenzhe Han, Qingyou Xia, Ping Zhao |
| المصدر: |
International Journal of Molecular Sciences; Volume 19; Issue 8; Pages: 2275 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2018 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
silkworm, antibacterial peptide, Gloverin, gram-negative bacteria, antibacterial mechanism |
| جغرافية الموضوع: |
agris |
| الوصف: |
Gloverin is one of the glycine-rich antimicrobial peptide exclusively found in Lepidoptera insects. It is generally activated through the innate immune system in insects. In this study, recombinant Gloverin2 from Bombyx mori (BmGlv2) was synthesized using a prokaryotic expression system. Circular dichroism spectroscopy showed that the recombinant BmGlv2 has random coil structure, which is relatively stable at the temperatures ranging from 15 to 82.5 °C. Antimicrobial activity analysis revealed that BmGlv2 significantly inhibited the growth of gram-negative bacteria, Escherichia coli JM109 and Pseudomonas putida, by disrupting cell integrity. Western blotting and immunofluorescence analyses suggested that BmGlv2 absorbed on the cell surface after incubation, which might be the first step in the antibacterial process. Our results also proved that the cell wall component lipopolysaccharides (LPS) induce a conformational change in BmGlv2 from a random coil to α-helix. Subsequently, α-helical BmGlv2 would recruit more BmGlv2 and form higher aggregation state. Collectively, these findings expand our understanding of antibacterial mechanism of BmGlv2. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Biochemistry; https://dx.doi.org/10.3390/ijms19082275 |
| DOI: |
10.3390/ijms19082275 |
| الاتاحة: |
https://doi.org/10.3390/ijms19082275 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.282C252E |
| قاعدة البيانات: |
BASE |