Academic Journal
Brain damage and hypoxia in an ovine fetal chronic cocaine model1
| العنوان: | Brain damage and hypoxia in an ovine fetal chronic cocaine model1 |
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| المؤلفون: | Laurini, R. N., Arbeille, B., Gemberg, C., Akoka, S., Locatelli, A., Lansac, J., Arbeille, P. |
| المصدر: | European Journal of Obstetrics, Gynecology, and Reproductive Biology, vol. 86, no. 1, pp. 15-22 |
| سنة النشر: | 1999 |
| المجموعة: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| مصطلحات موضوعية: | Animals/Apoptosis/Brain Damage,Chronic/chemically induced/etiology/Pathology/Cell Nucleus/Cocaine/administration & dosage/Cytoplasm/Disease Models,Animal/Female/Fetal Hypoxia/complications/Glial Fibrillary Acidic Protein/analysis/Microscopy,Electron/Neurons/Pregnancy/Sheep |
| الوصف: | OBJECTIVE: To assess the development of brain damage in an ovine fetal chronic cocaine model. To evaluate the effect of isolated hypoxic tests on this model and to correlate hemodynamic findings (brain-sparing effect) following fetal hypoxia and the occurrence of brain damage. STUDY DESIGN: Fifteen ewes were divided into a control group (n=7) and a cocaine treated group (n=8). From day 65 to day 134 the cocaine treated animals received a daily (5 days per week) intramuscular injection (2 mg/kg cocaine) and the control animals a placebo injection (2 ml of isotonic solution). Both groups underwent hypoxic tests (cord compression (3 min) and aortic compression (1 min)) at 90 and 134 days. In addition, anesthesia for magnetic resonance imaging (MRI) examination was carried out at 125 days. Fetal blood samples were collected during both series of hypoxic tests and the cerebral and umbilical flows were monitored by Doppler. Samples from 25 brains (control n = 10; cocaine n= 15) were processed for light and electron microscopic examination. Quantification of brain damage was done on semithin sections from six areas of cortex and germinal matrix on each fetus. RESULTS: Similar forms of brain damage (selective neuronal loss limited to the parasaggital cortex, striatum, hippocampus and Purkinje cells) was present in both groups but lesions were more frequent in the cocaine treated group as shown by quantitative analysis for the proportion of abnormal capillaries (65% vs. 35%), capillary edema (61% vs. 34%) and abnormal neurons showing delayed neuronal degeneration (DND) (66% vs. 36%) in the cocaine and control group respectively. There was no significant difference in immunoreactivity for glial fibrillary acidic protein (GFAP) but it was more marked in the cerebellum of cocaine treated animals. Fetal blood samples showed a moderate sustained hypoxia and Doppler findings demonstrated the presence of a brain sparing effect associated with increased uterine and umbilical vascular resistance in the cocaine treated group. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/10471137; info:eu-repo/semantics/altIdentifier/pissn/0301-2115; https://serval.unil.ch/notice/serval:BIB_973884AAC980 |
| DOI: | 10.1016/S0301-2115(99)00036-6 |
| الاتاحة: | https://serval.unil.ch/notice/serval:BIB_973884AAC980 https://doi.org/10.1016/S0301-2115(99)00036-6 |
| رقم الانضمام: | edsbas.27B31DEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/S0301-2115(99)00036-6 |
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