Book
Construction and analysis of full-length and normalized cDNA libraries from citrus
| العنوان: | Construction and analysis of full-length and normalized cDNA libraries from citrus |
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| المؤلفون: | Marques, M. Carmen, Perez Amador, Miguel Angel |
| المساهمون: | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, Ministerio de Ciencia y Tecnología |
| بيانات النشر: | Springer New York |
| سنة النشر: | 2012 |
| المجموعة: | Universitat Politécnica de Valencia: RiuNet / Politechnical University of Valencia |
| مصطلحات موضوعية: | Library, cDNA, Citrus, Full-length, Normalized, SMART, DSN nuclease, Gateway technology |
| الوصف: | [EN] We have developed an integrated method to generate a normalized cDNA collection enriched in full-length and rare transcripts from citrus, using different species and multiple tissues and developmental stages. Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. In this regard, the availability of full-length cDNA clones facilitates functional analysis of the corresponding genes enabling manipulation of their expression and the generation of a variety of tagged versions of the native protein. The development of full-length cDNA sequences has the power to improve the quality of genome annotation, as well as provide tools for functional characterization of genes ; The authors would like to thank to all participants in the Spanish Citrus Functional Genomic Project, specially to Drs. Javier Forment, Jose Gadea, and Vicente Conejero. This work was funded by grants from the Spanish Government GEN2001-4885-CO5-01 and GEN2001-4885-CO5-02. ; Marques, MC.; Perez Amador, MA. (2012). Construction and analysis of full-length and normalized cDNA libraries from citrus. Functional Genomics: Methods and Protocols. 815:51-65. https://doi.org/10.1007/978-1-61779-424-7_5 ; S ; 51 ; 65 ; 815 ; Carninci P, Kvam C, Kitamura A, Ohsumi T, Okazaki Y, Itoh M, Kamiya M, Shibata K, Sasaki N, Izawa M, Muramatsu M, Hayashizaki Y, and Schneider C (1996) High-efficiency full-length cDNA cloning by biotinylated CAP trapper. Genomics 37 327–336. ; Carninci P, Shibata Y, Hayatsu N, Sugahara Y, Shibata K, Itoh M, Konno H, Okazaki Y, Muramatsu M, and Hayashizaki Y (2000) Normalization and subtraction of CAP-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes. Genome Res. 10, 1617–1630. ; Suzuki Y, and Sugano S (2003) Construction of a full-length enriched and a 5’-end enriched cDNA library using the Oligo-capping method. Methods Mol. Biol. 221 73–91. ; Clepet C, Le ... |
| نوع الوثيقة: | book part article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-1-61779-423-0 978-1-61779-424-7 1-61779-423-6 1-61779-424-4 |
| تدمد: | 1064-3745 |
| Relation: | Functional Genomics: Methods and Protocols; Methods in Molecular Biology;815; info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05-01/ES/ESTUDIO DE LA RESPUESTA A ESTRESES BIOTICOS DE CITRICOS MEDIANTE GENOMICA FUNCIONAL/; info:eu-repo/grantAgreement/MICYT//GEN2001-4885-C05-02/ES/ESTUDIO DEL DESARROLLO VEGETATIVO Y REPRODUCTIVO DE CITRICOS MEDIANTE GENOMICA FUNCIONAL/; https://doi.org/10.1007/978-1-61779-424-7_5; urn:isbn:978-1-61779-423-0; urn:isbn:978-1-61779-424-7; urn:issn:1064-3745; http://hdl.handle.net/10251/83428 |
| DOI: | 10.1007/978-1-61779-424-7_5 |
| الاتاحة: | http://hdl.handle.net/10251/83428 https://doi.org/10.1007/978-1-61779-424-7_5 |
| Rights: | http://rightsstatements.org/vocab/InC/1.0/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.27B203C6 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781617794230 9781617794247 1617794236 1617794244 |
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| تدمد: | 10643745 |
| DOI: | 10.1007/978-1-61779-424-7_5 |