Academic Journal
Detection of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Pools of Sera Negative for Antibodies to HIV-1 and HIV-2
| العنوان: | Detection of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Pools of Sera Negative for Antibodies to HIV-1 and HIV-2 |
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| المؤلفون: | Morandi, Pierre-Alain, Schockmel, Gérard A., Yerly, Sabine, Burgisser, Philippe, Erb, Peter, Matter, Lukas, Sitavanc, Radan, Perrin, Luc |
| المصدر: | Journal of Clinical Microbiology ; volume 36, issue 6, page 1534-1538 ; ISSN 0095-1137 1098-660X |
| بيانات النشر: | American Society for Microbiology |
| سنة النشر: | 1998 |
| الوصف: | A total of 234 pools were prepared from 10,692 consecutive serum samples negative for antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 collected at five virological laboratories (average pool size, 45 serum samples). Pools were screened for the presence of HIV-1 RNA by a modified commercial assay (Amplicor HIV-1 Monitor test) which included an additional polyethylene glycol (PEG) precipitation step prior to purification of viral RNA (PEG Amplicor assay). The sensitivity of this assay for HIV-1 RNA detection in individual serum samples within pools matches that of standard commercial assays for individual serum samples, i.e., 500 HIV-1 RNA copies per ml. Five pools were identified as positive, and each one contained one antibody-negative, HIV-1 RNA-positive serum sample, corresponding to an average of 1 infected sample per 2,138 serum samples. Retrospective analysis revealed that the five HIV-1 RNA-positive specimens originated from individuals who had symptomatic primary HIV-1 infection at the time of sample collection and who were also positive for p24 antigenemia. We next assessed the possibility of performing the prepurification step by high-speed centrifugation (50,000 × g for 80 min) of 1.5-ml pools containing 25 μl of 60 individual serum samples, of which only 1 contained HIV-1 RNA (centrifugation Amplicor assay). The sensitivity of this assay also matches the sensitivities of standard commercial assays for HIV-1 RNA detection in individual serum samples. The results demonstrate that both assays with pooled sera can be applied to the screening of large numbers of serum samples in a time- and cost-efficient manner. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1128/jcm.36.6.1534-1538.1998 |
| DOI: | 10.1128/JCM.36.6.1534-1538.1998 |
| الاتاحة: | http://dx.doi.org/10.1128/jcm.36.6.1534-1538.1998 https://journals.asm.org/doi/pdf/10.1128/JCM.36.6.1534-1538.1998 |
| Rights: | https://journals.asm.org/non-commercial-tdm-license |
| رقم الانضمام: | edsbas.279935D4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/jcm.36.6.1534-1538.1998 |
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