Academic Journal
Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport.
| العنوان: | Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport. |
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| المؤلفون: | Lambers, T.T., Mahieu, F., Oancea, E., Hoofd, L.J.C., Lange, F. de, Mensenkamp, A.R., Voets, T., Nilius, B., Clapham, D.E., Hoenderop, J.G.J., Bindels, R.J.M. |
| المصدر: | EMBO Journal, 25, 13, pp. 2978-88 |
| سنة النشر: | 2006 |
| المجموعة: | Radboud University: DSpace |
| مصطلحات موضوعية: | IGMD 3: Genomic disorders and inherited multi-system disorders, IGMD 9: Renal disorder, NCMLS 5: Membrane transport and intracellular motility, ONCOL 1: Hereditary cancer and cancer-related syndromes, UMCN 5.4: Renal disorders |
| الوصف: | Contains fulltext : 49350.pdf (publisher's version ) (Closed access) ; In Ca(2+)-transporting epithelia, calbindin-D(28K) (CaBP(28K)) facilitates Ca(2+) diffusion from the luminal Ca(2+) entry side of the cell to the basolateral side, where Ca(2+) is extruded into the extracellular compartment. Simultaneously, CaBP(28K) provides protection against toxic high Ca(2+) levels by buffering the cytosolic Ca(2+) concentration ([Ca(2+)](i)) during high Ca(2+) influx. CaBP(28K) consistently colocalizes with the epithelial Ca(2+) channel TRPV5, which constitutes the apical entry step in renal Ca(2+)-transporting epithelial cells. Here, we demonstrate using protein-binding analysis, subcellular fractionation and evanescent-field microscopy that CaBP(28K) translocates towards the plasma membrane and directly associates with TRPV5 at a low [Ca(2+)](i). (45)Ca(2+) uptake measurements, electrophysiological recordings and transcellular Ca(2+) transport assays of lentivirus-infected primary rabbit connecting tubule/distal convolute tubule cells revealed that associated CaBP(28K) tightly buffers the flux of Ca(2+) entering the cell via TRPV5, facilitating high Ca(2+) transport rates by preventing channel inactivation. In summary, CaBP(28K) acts in Ca(2+)-transporting epithelia as a dynamic Ca(2+) buffer, regulating [Ca(2+)] in close vicinity to the TRPV5 pore by direct association with the channel. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | http://hdl.handle.net/2066/49350; https://doi.org/10.1038/sj.emboj.7601186 |
| DOI: | 10.1038/sj.emboj.7601186 |
| الاتاحة: | http://hdl.handle.net/2066/49350 https://doi.org/10.1038/sj.emboj.7601186 |
| رقم الانضمام: | edsbas.26CF178D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/sj.emboj.7601186 |
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