Academic Journal
Genome-Wide Association Mapping and Gene Expression Analysis Reveal the Negative Role of OsMYB21 in Regulating Bacterial Blight Resistance in Rice
| العنوان: | Genome-Wide Association Mapping and Gene Expression Analysis Reveal the Negative Role of OsMYB21 in Regulating Bacterial Blight Resistance in Rice |
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| المؤلفون: | Yang, Wu, Zhao, Junliang, Zhang, Shaohong, Chen, Luo, Yang, Tifeng, Dong, Jingfang, Fu, Hua, Ma, Yamei, Zhou, Lian, Wang, Jian, Liu, Wei, Liu, Qing, Liu, Bin |
| المساهمون: | National Key Research and Development Project, Innovation Team Project of Guangdong Modern Agricultural Industrial System, Key Areas Research Projects of Guangdong Province, Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science, Natural Science Foundation of Guangdong Province |
| المصدر: | Rice ; volume 14, issue 1 ; ISSN 1939-8425 1939-8433 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| الوصف: | Background Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae ( Xoo ), is one of the most devastating diseases in rice all over the world. Due to the diversity and rapid evolution of Xoo , identification and use of the non-race specific quantitative resistance QTLs has been considered the preferred strategy for effective control of this disease. Although numerous QTLs for BB resistance have been identified, they haven’t been effectively used for improvement of BB resistance in rice due to their small effects and lack of knowledge on the function of genes underlying the QTLs. Results In the present study, a genome-wide association study of BB resistance was performed in a rice core collection from South China. A total of 17 QTLs were identified to be associated with BB resistance. Among them, 13 QTLs were newly identified in the present study and the other 4 QTLs were co-localized with the previously reported QTLs or Xa genes that confer qualitative resistance to Xoo strains. Particularly, the qBBR11–4 on chromosome 11 explained the largest phenotypic variation in this study and was co-localized with the previously identified QTLs for BB and bacterial leaf streak (BLS) resistance against diverse strains in three studies, suggesting its broad-spectrum resistance and potential value in rice breeding. Through combined analysis of differential expression and annotations of the predicted genes within qBBR11–4 between two sets of rice accessions selected based on haplotypes and disease phenotypes, we identified the transcription factor OsMYB21 as the candidate gene for qBBR11–4 . The OsMYB21 overexpressing plants exhibited decreased resistance to bacterial blight, accompanied with down-regulation of several defense-related genes compared with the wild-type plants. Conclusion The results suggest that OsMYB21 negatively regulates bacterial blight resistance in rice, and this gene can be a promising target in rice breeding by using the gene editing method. In addition, the potential candidate genes ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12284-021-00501-z |
| DOI: | 10.1186/s12284-021-00501-z.pdf |
| DOI: | 10.1186/s12284-021-00501-z/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12284-021-00501-z https://link.springer.com/content/pdf/10.1186/s12284-021-00501-z.pdf https://link.springer.com/article/10.1186/s12284-021-00501-z/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.26A0166E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12284-021-00501-z |
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