Academic Journal
Molecular characterization of antibiotic resistance in Escherichia coli strains from a dairy cattle farm and its surroundings
| العنوان: | Molecular characterization of antibiotic resistance in Escherichia coli strains from a dairy cattle farm and its surroundings |
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| المؤلفون: | Navajas-Benito, E.V., Alonso, C.A., Sanz, S., Olarte, C., Martínez-Olarte, R., Hidalgo-Sanz, S., Somalo, S., Torres, C. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | air, cattle, farm environment, resistance genes |
| الوصف: | BACKGROUND: This study describes the phenotypic and genotypic characteristics of 78 genetically different Escherichia coli recovered from air and exudate samples of a dairy cattle farm and its surroundings in Spain, in order to gain insight into the flow of antimicrobial resistance through the environment and food supply. RESULTS: Antimicrobial resistance was detected in 21.8% of the 78 E. coli isolates analyzed (resistance for at least one of the 14 agents tested). The highest resistance rates were recorded for ampicillin, nalidixic acid, trimethoprim/sulfamethoxazole and tetracycline. The resistance genes detected were as follows (antibiotic (number of resistant strains), gene (number of strains)): ampicillin (9), blaTEM-1 (6); tetracycline (15), tet(A) (7), tet(B) (4), tet(A) + tet(B) (1); chloramphenicol (5), cmlA (2), floR (2); trimethoprim/sulfamethoxazole (10), sul2 (4), sul1 (3), sul3 (2), sul1 + sul2 (1); gentamicin-tobramycin (1), ant(2) (1). About 14% of strains showed a multidrug-resistant phenotype and, of them, seven strains carried class 1 integrons containing predominantly the dfrA1-aadA1 array. One multidrug-resistant strain was found in both inside and outside air, suggesting that the airborne spread of multidrug-resistant bacteria from the animal housing facilities to the surroundings is feasible. CONCLUSIONS: This study gives a genetic background of the antimicrobial resistance problem in a dairy cattle farm and shows that air can act as a source for dissemination of antimicrobial-resistant bacteria. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0022-5142 |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000387349900047; info:eu-repo/semantics/altIdentifier/pissn/0022-5142; Molecular characterization of antibiotic resistance in Escherichia coli strains from a dairy cattle farm and its surroundings, 2017, vol. 97, núm. 1, pág. 362-365; https://investigacion.unirioja.es/documentos/5bbc6881b750603269e80a34; urn:issn:0022-5142 |
| DOI: | 10.1002/JSFA.7709 |
| الاتاحة: | https://investigacion.unirioja.es/documentos/5bbc6881b750603269e80a34 https://doi.org/10.1002/JSFA.7709 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.269ED0D8 |
| قاعدة البيانات: | BASE |
| تدمد: | 00225142 |
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| DOI: | 10.1002/JSFA.7709 |