Academic Journal
Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities
| العنوان: | Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities |
|---|---|
| المؤلفون: | Perinelli M., Guerrini R., Albanese V., Marchetti N., Bellotti D., Gentili S., Tegoni M., Remelli M. |
| المساهمون: | Perinelli, M., Guerrini, R., Albanese, V., Marchetti, N., Bellotti, D., Gentili, S., Tegoni, M., Remelli, M. |
| بيانات النشر: | Elsevier Inc. |
| سنة النشر: | 2020 |
| المجموعة: | Università di Parma: CINECA IRIS |
| مصطلحات موضوعية: | Branched peptide, Copper(II), Cyclam scaffold, Metal complexe, Peptide welding technology, Solution equilibria |
| الوصف: | The two branched peptides (AAHAWG)4-PWT2 and (HAWG)4-PWT2 where synthesized by mounting linear peptides on a cyclam-based scaffold (PWT2), provided with four maleimide chains, through a thio-Michael reaction. The purpose of this study was primarily to verify if the two branched ligands had a Cu(II) coordination behavior reproducing that of the single-chain peptides, namely AAHAWG-NH2, which bears an Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) Motif, and HAWG-NH2, which presents a His residue as the N-terminal amino acid, in a wide pH range. The study of Cu(II) binding was performed by potentiometric, spectroscopic (UV–vis absorption, CD, fluorescence) and ESI-MS techniques. ATCUN-type ligands ((AAHAWG)4-PWT2 and AAHAWG-NH2) were confirmed to bind one Cu(II) per peptide fragment at both pH 7.4 and pH 9.0, with a [NH2, 2N−, NIm] coordination mode. On the other hand, the ligand HAWG-NH2 forms a [CuL2]2+ species at neutral pH, while, at pH 9, the formation of 1:2 Cu(II):ligand adducts is prevented by amidic nitrogen deprotonation and coordination, to give rise solely to 1:1 species. Conversely, Cu(II) binding to (HAWG)4-PWT2 resulted in the formation of 1:2 copper:peptide chain also at pH 9: hence, through the latter branched peptide we obtained, at alkaline pH, the stabilization of a specific Cu(II) coordination mode which results unachievable using the corresponding single-chain peptide. This behavior could be explained in terms of high local peptide concentration on the basis of the speciation of the Cu(II)/single-chain peptide systems. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/wos/WOS:000522119300008; volume:205; firstpage:110980; journal:JOURNAL OF INORGANIC BIOCHEMISTRY; http://hdl.handle.net/11381/2886221; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85077661872 |
| DOI: | 10.1016/j.jinorgbio.2019.110980 |
| الاتاحة: | http://hdl.handle.net/11381/2886221 https://doi.org/10.1016/j.jinorgbio.2019.110980 |
| رقم الانضمام: | edsbas.2692555A |
| قاعدة البيانات: | BASE |
| ResultId |
1 |
|---|---|
| Header |
edsbas BASE edsbas.2692555A 835 3 Academic Journal academicJournal 835.017944335938 |
| PLink |
https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&scope=site&db=edsbas&AN=edsbas.2692555A&custid=s6537998&authtype=sso |
| FullText |
Array
(
[Availability] => 0
)
Array ( [0] => Array ( [Url] => http://hdl.handle.net/11381/2886221# [Name] => EDS - BASE [Category] => fullText [Text] => View record in BASE [MouseOverText] => View record in BASE ) ) |
| Items |
Array
(
[Name] => Title
[Label] => Title
[Group] => Ti
[Data] => Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities
)
Array ( [Name] => Author [Label] => Authors [Group] => Au [Data] => <searchLink fieldCode="AR" term="%22Perinelli+M%2E%22">Perinelli M.</searchLink><br /><searchLink fieldCode="AR" term="%22Guerrini+R%2E%22">Guerrini R.</searchLink><br /><searchLink fieldCode="AR" term="%22Albanese+V%2E%22">Albanese V.</searchLink><br /><searchLink fieldCode="AR" term="%22Marchetti+N%2E%22">Marchetti N.</searchLink><br /><searchLink fieldCode="AR" term="%22Bellotti+D%2E%22">Bellotti D.</searchLink><br /><searchLink fieldCode="AR" term="%22Gentili+S%2E%22">Gentili S.</searchLink><br /><searchLink fieldCode="AR" term="%22Tegoni+M%2E%22">Tegoni M.</searchLink><br /><searchLink fieldCode="AR" term="%22Remelli+M%2E%22">Remelli M.</searchLink> ) Array ( [Name] => Author [Label] => Contributors [Group] => Au [Data] => Perinelli, M.<br />Guerrini, R.<br />Albanese, V.<br />Marchetti, N.<br />Bellotti, D.<br />Gentili, S.<br />Tegoni, M.<br />Remelli, M. ) Array ( [Name] => Publisher [Label] => Publisher Information [Group] => PubInfo [Data] => Elsevier Inc. ) Array ( [Name] => DatePubCY [Label] => Publication Year [Group] => Date [Data] => 2020 ) Array ( [Name] => Subset [Label] => Collection [Group] => HoldingsInfo [Data] => Università di Parma: CINECA IRIS ) Array ( [Name] => Subject [Label] => Subject Terms [Group] => Su [Data] => <searchLink fieldCode="DE" term="%22Branched+peptide%22">Branched peptide</searchLink><br /><searchLink fieldCode="DE" term="%22Copper%28II%29%22">Copper(II)</searchLink><br /><searchLink fieldCode="DE" term="%22Cyclam+scaffold%22">Cyclam scaffold</searchLink><br /><searchLink fieldCode="DE" term="%22Metal+complexe%22">Metal complexe</searchLink><br /><searchLink fieldCode="DE" term="%22Peptide+welding+technology%22">Peptide welding technology</searchLink><br /><searchLink fieldCode="DE" term="%22Solution+equilibria%22">Solution equilibria</searchLink> ) Array ( [Name] => Abstract [Label] => Description [Group] => Ab [Data] => The two branched peptides (AAHAWG)4-PWT2 and (HAWG)4-PWT2 where synthesized by mounting linear peptides on a cyclam-based scaffold (PWT2), provided with four maleimide chains, through a thio-Michael reaction. The purpose of this study was primarily to verify if the two branched ligands had a Cu(II) coordination behavior reproducing that of the single-chain peptides, namely AAHAWG-NH2, which bears an Amino Terminal Cu(II)- and Ni(II)-Binding (ATCUN) Motif, and HAWG-NH2, which presents a His residue as the N-terminal amino acid, in a wide pH range. The study of Cu(II) binding was performed by potentiometric, spectroscopic (UV–vis absorption, CD, fluorescence) and ESI-MS techniques. ATCUN-type ligands ((AAHAWG)4-PWT2 and AAHAWG-NH2) were confirmed to bind one Cu(II) per peptide fragment at both pH 7.4 and pH 9.0, with a [NH2, 2N−, NIm] coordination mode. On the other hand, the ligand HAWG-NH2 forms a [CuL2]2+ species at neutral pH, while, at pH 9, the formation of 1:2 Cu(II):ligand adducts is prevented by amidic nitrogen deprotonation and coordination, to give rise solely to 1:1 species. Conversely, Cu(II) binding to (HAWG)4-PWT2 resulted in the formation of 1:2 copper:peptide chain also at pH 9: hence, through the latter branched peptide we obtained, at alkaline pH, the stabilization of a specific Cu(II) coordination mode which results unachievable using the corresponding single-chain peptide. This behavior could be explained in terms of high local peptide concentration on the basis of the speciation of the Cu(II)/single-chain peptide systems. ) Array ( [Name] => TypeDocument [Label] => Document Type [Group] => TypDoc [Data] => article in journal/newspaper ) Array ( [Name] => Language [Label] => Language [Group] => Lang [Data] => English ) Array ( [Name] => NoteTitleSource [Label] => Relation [Group] => SrcInfo [Data] => info:eu-repo/semantics/altIdentifier/wos/WOS:000522119300008; volume:205; firstpage:110980; journal:JOURNAL OF INORGANIC BIOCHEMISTRY; http://hdl.handle.net/11381/2886221; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85077661872 ) Array ( [Name] => DOI [Label] => DOI [Group] => ID [Data] => 10.1016/j.jinorgbio.2019.110980 ) Array ( [Name] => URL [Label] => Availability [Group] => URL [Data] => http://hdl.handle.net/11381/2886221<br />https://doi.org/10.1016/j.jinorgbio.2019.110980 ) Array ( [Name] => AN [Label] => Accession Number [Group] => ID [Data] => edsbas.2692555A ) |
| RecordInfo |
Array
(
[BibEntity] => Array
(
[Identifiers] => Array
(
[0] => Array
(
[Type] => doi
[Value] => 10.1016/j.jinorgbio.2019.110980
)
)
[Languages] => Array
(
[0] => Array
(
[Text] => English
)
)
[Subjects] => Array
(
[0] => Array
(
[SubjectFull] => Branched peptide
[Type] => general
)
[1] => Array
(
[SubjectFull] => Copper(II)
[Type] => general
)
[2] => Array
(
[SubjectFull] => Cyclam scaffold
[Type] => general
)
[3] => Array
(
[SubjectFull] => Metal complexe
[Type] => general
)
[4] => Array
(
[SubjectFull] => Peptide welding technology
[Type] => general
)
[5] => Array
(
[SubjectFull] => Solution equilibria
[Type] => general
)
)
[Titles] => Array
(
[0] => Array
(
[TitleFull] => Cu(II) coordination to His-containing linear peptides and related branched ones: Equalities and diversities
[Type] => main
)
)
)
[BibRelationships] => Array
(
[HasContributorRelationships] => Array
(
[0] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Perinelli M.
)
)
)
[1] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Guerrini R.
)
)
)
[2] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Albanese V.
)
)
)
[3] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Marchetti N.
)
)
)
[4] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Bellotti D.
)
)
)
[5] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gentili S.
)
)
)
[6] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Tegoni M.
)
)
)
[7] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Remelli M.
)
)
)
[8] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Perinelli, M.
)
)
)
[9] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Guerrini, R.
)
)
)
[10] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Albanese, V.
)
)
)
[11] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Marchetti, N.
)
)
)
[12] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Bellotti, D.
)
)
)
[13] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Gentili, S.
)
)
)
[14] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Tegoni, M.
)
)
)
[15] => Array
(
[PersonEntity] => Array
(
[Name] => Array
(
[NameFull] => Remelli, M.
)
)
)
)
[IsPartOfRelationships] => Array
(
[0] => Array
(
[BibEntity] => Array
(
[Dates] => Array
(
[0] => Array
(
[D] => 01
[M] => 01
[Type] => published
[Y] => 2020
)
)
[Identifiers] => Array
(
[0] => Array
(
[Type] => issn-locals
[Value] => edsbas
)
)
)
)
)
)
)
|
| IllustrationInfo |